ArticlePlant physiology2017
Validating Genome-Wide Association Candidates Controlling Quantitative Variation in Nodulation.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 86 citations in OpenAlex.
- Environmental Association Analyses in Plants and the Influence of Methodological Decisions.Molecular ecology · 2026Review
- Population genomics of yellow-eyed penguins uncovers subspecies divergence and candidate genes linked to respiratory distress syndrome.Nature ecology & evolution · 2026Article
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- Molecular Network Analysis in Model and Non-Model Legumes: Challenges in Omics Data Interpretation Across Species, with a Focus onPlants (Basel, Switzerland) · 2025Review
- CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects.Plant molecular biology · 2025Review
- Unveiling novel QTLs for nitrogen use efficiency in temperate Japonica rice.BMC plant biology · 2025Article
- Partners in root nodule symbiosis respond uniquely to heavy metal stresses in a host genotype-dependent manner.Scientific reports · 2025Article
- Highly Efficient Generation of Germline Mutations Using CRISPR/Cas9 in the Speckled Wood ButterflyEcology and evolution · 2025Article
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- Genome editing of an oxalyl-CoA synthetase gene in Lathyrus sativus reveals its role in oxalate metabolism.Plant cell reports · 2024Article
- The Hessian fly resistance gene HvRHF1 is localized in an NBS-LRR gene cluster in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- CRISPR/Cas9-Mediated Generation of Mutant Lines inBiology · 2024Article
- Species-wide genomics of kākāpō provides tools to accelerate recovery.Nature ecology & evolution · 2023Article
- Review
- Mapping Genetic Variation in Arabidopsis in Response to Plant Growth-Promoting BacteriumMicroorganisms · 2023Article
- Gene-by-environment interactions in plants: Molecular mechanisms, environmental drivers, and adaptive plasticity.The Plant cell · 2023Review
- Article
- Genetic architecture of the response ofFrontiers in plant science · 2023Article
- Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Life (Basel, Switzerland) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide association (GWA) studies offer the opportunity to identify genes that contribute to naturally occurring variation in quantitative traits. However, GWA relies exclusively on statistical association, so functional validation is necessary to make strong claims about gene function. We used a combination of gene-disruption platforms (Tnt1 retrotransposons, hairpin RNA-interference constructs, and CRISPR/Cas9 nucleases) together with randomized, well-replicated experiments to evaluate the function of genes that an earlier GWA study in Medicago truncatula had identified as candidates contributing to variation in the symbiosis between legumes and rhizobia. We evaluated ten candidate genes found in six clusters of strongly associated single nucleotide polymorphisms, selected on the basis of their strength of statistical association, proximity to annotated gene models, and root or nodule expression. We found statistically significant effects on nodule production for three candidate genes, each validated in two independent mutants. Annotated functions of these three genes suggest their contributions to quantitative variation in nodule production occur through processes not previously connected to nodulation, including phosphorous supply and salicylic acid-related defense response. These results demonstrate the utility of GWA combined with reverse mutagenesis technologies to discover and validate genes contributing to naturally occurring variation in quantitative traits. The results highlight the potential for GWA to complement forward genetics in identifying the genetic basis of ecologically and economically important traits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.