ArticleProceedings of the National Academy of Sciences of the United States of America2017
Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
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- A nanobody-based degron system for targeted protein knockdown in Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- The Proteome ofProteomes · 2026Article
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- Optogenetic control of cAMP oscillations reveals frequency-selective transcription factor dynamics in Dictyostelium.Development (Cambridge, England) · 2025Article
- Emerging roles for diguanylate cyclase during the evolution of soma in dictyostelia.BMC ecology and evolution · 2023Article
- Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP.Biology open · 2023Article
- Visualization of c-di-GMP in multicellularFrontiers in cell and developmental biology · 2023Article
- Evolution of a novel cell type in Dictyostelia required gene duplication of a cudA-like transcription factor.Current biology : CB · 2022Article
- Novel RNAseq-Informed Cell-type Markers and Their Regulation Alter Paradigms ofFrontiers in cell and developmental biology · 2022Article
- Review
- From environmental sensing to developmental control: cognitive evolution in dictyostelid social amoebas.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.Autophagy · 2021Article
- Loss of PIKfyve Causes Transdifferentiation ofFrontiers in cell and developmental biology · 2021Article
- CRISPR Toolbox for Genome Editing inFrontiers in cell and developmental biology · 2021Article
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
- Evolution of multicellularity in Dictyostelia.The International journal of developmental biology · 2019Review
- Cell-type specific RNA-Seq reveals novel roles and regulatory programs for terminally differentiated Dictyostelium cells.BMC genomics · 2018Article
- Improved annotation with de novo transcriptome assembly in four social amoeba species.BMC genomics · 2017Article
- Evolution of developmental signalling in Dictyostelid social amoebas.Current opinion in genetics & development · 2016Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Coordination of cell movement with cell differentiation is a major feat of embryonic development. The Dictyostelium stalk always forms at the organizing tip, by a mechanism that is not understood. We previously reported that cyclic diguanylate (c-di-GMP), synthesized by diguanylate cyclase A (DgcA), induces stalk formation. Here we used transcriptional profiling of dgca- structures to identify target genes for c-di-GMP, and used these genes to investigate the c-di-GMP signal transduction pathway. We found that knockdown of cAMP-dependent protein kinase (PKA) activity in prestalk cells reduced stalk gene induction by c-di-GMP, whereas PKA activation bypassed the c-di-GMP requirement for stalk gene expression. c-di-GMP caused a persistent increase in cAMP, which still occurred in mutants lacking the adenylate cyclases ACG or ACR, or the cAMP phosphodiesterase RegA. However, both inhibition of adenylate cyclase A (ACA) with SQ22536 and incubation of a temperature-sensitive ACA mutant at the restrictive temperature prevented c-di-GMP-induced cAMP synthesis as well as c-di-GMP-induced stalk gene transcription. ACA produces the cAMP pulses that coordinate Dictyostelium morphogenetic cell movement and is highly expressed at the organizing tip. The stalk-less dgca- mutant regained its stalk by expression of a light-activated adenylate cyclase from the ACA promoter and exposure to light, indicating that cAMP is also the intermediate for c-di-GMP in vivo. Our data show that the more widely expressed DgcA activates tip-expressed ACA, which then acts on PKA to induce stalk genes. These results explain why stalk formation in Dictyostelia always initiates at the site of the morphogenetic organizer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.