ArticleBMC veterinary research2017
Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Influence of Manual Double-Spin Preparation Protocols on the Cellular and Growth Factor Composition of Equine Platelet-Rich Plasma.Animals : an open access journal from MDPI · 2026Article
- Investigation into the Short-Term Effects of Intra-Articular Allogenic PRP in the Equine LPS Model of Joint Inflammation: A Randomised Control Trial.Animals : an open access journal from MDPI · 2026Article
- In Vitro Effects of PRP, Ozonized PRP, Hyaluronic Acid, Paracetamol, and Polyacrylamide on Equine Synovial Fluid-Derived Mesenchymal Stem Cells.Life (Basel, Switzerland) · 2025Article
- Equine bone marrow aspirate and bone marrow aspirate concentrate are enriched with interleukin-1 receptor antagonist protein.American journal of veterinary research · 2025Article
- Medium-term storage of platelet-derived orthobiologics: a feasible alternative for equine practice.Frontiers in veterinary science · 2025Article
- Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.International journal of veterinary science and medicine · 2024Article
- Equine peripheral blood CD14Veterinary immunology and immunopathology · 2023Article
- Regenerative Medicine for Equine Musculoskeletal Diseases.Animals : an open access journal from MDPI · 2021Review
- A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade.Frontiers in veterinary science · 2021Review
- Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma.The Journal of veterinary medical science · 2020Article
- Platelet-rich plasma (PRP) therapy: An approach in reproductive medicine based on successful animal models.Animal reproduction · 2020Article
- Platelet and Leukocyte Concentration in Equine Autologous Conditioned Plasma Are Inversely Distributed by Layer and Are Not Affected by Centrifugation Rate.Frontiers in veterinary science · 2020Article
- The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months.BMC veterinary research · 2019Article
- An investigation of the equine epidermal growth factor system during hyperinsulinemic laminitis.PloS one · 2019Article
- Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma.Veterinary medicine international · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe increasing interest in platelet-rich plasma (PRP) based therapies is as yet accompanied by inconsistent information regarding nearly all aspects of handling and application. Among these storage stability of processed platelet-rich products may be the basis for a more flexible application mode. The objective of this study was (1) to estimate the storage stability of growth factors platelet derived growth factor BB (PDGF-BB) and transforming growth factor ß1 (TGF-ß1) in both, a single-step softspin centrifugation-based pure-PRP (P-PRP, ACP®), and a gravity filtration system-based leukocyte-rich-PRP (L-PRP, E-PET), over a six hours time span after preparation at room temperature and (2) to identify possible factors influencing these growth factor concentrations in an equine model.
resultsGrowth factor concentrations remained stable over the entire investigation period in L-PRP as well as P-PRP preparations revealing a mean of 3569 pg/ml PDGF-BB for E-PET and means of 1276 pg/ml PDGF-BB and 5086 pg/ml TGF-ß1 for ACP®. Pearson correlations yielded no significant impact of whole blood platelet (PLT), white blood cell (WBC) and red blood cell (RBC) counts on resulting cytokine values. In case of ACP® no significant dependencies between PLT, WBC and RBC counts of the processed platelet-rich product and resulting cytokine content occurred with exception of TGF-ß1 concentrations showing a strong correlation with the WBC content. PDGF-BB content of E-PET preparations showed a strong positive correlation with PLT and a strong negative with WBC of these preparations but not with RBC.
conclusionsL-PRP ad modum E-PET and P-PRP ad modum ACP® are applicable over at least a six hours time span at room temperature without loss of growth factor content. Based on the results of this study factors influencing the resulting growth factor concentrations still remain questionable. Additional studies implicating a further standardization of preparation protocols are necessary to identify consistent impact on cytokine content after PRP processing.
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