Evidence map›Paper›PMID 28056965›Full record

ArticleBMC veterinary research2017

Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014.

Seetha Jaganathan King, Peck Toung Ooi, Lai Yee Phang, Zeenathul Nazariah Binti Allaudin, Wei Hoong Loh, Chiou Yan Tee, Shiao Pau How, Lai Siong Yip, Pow Yoon Choo, Ban Keong Lim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 82% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Seetha Jaganathan KingDepartment of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.
Peck Toung OoiDepartment of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia. ooi@upm.edu.my.
Lai Yee PhangDepartment of Biotechnology, Faculty of Biotechnology & Molecular Science, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.
Zeenathul Nazariah Binti AllaudinDepartment of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.
Wei Hoong LohVet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Chiou Yan TeeVet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Shiao Pau HowVet Food Agro Diagnostic Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Lai Siong YipVet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Pow Yoon ChooVet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Ban Keong LimVet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
Universiti Putra Malaysia · MYJabatan Perkhidmatan Awam Malaysia · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPorcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S. alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.

methodsLungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6.06®.

resultsAnalysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.

conclusionsOur study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.

Indexed as

PhylogenyAnimalsGene Expression Regulation, ViralMalaysiaPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSwineTime FactorsViral Envelope Proteinsglycoprotein 5, PRRSVViral Envelope ProteinsGenetic characterizationORF5 geneORF7 genePorcine reproductive and respiratory syndrome virusPRRSV

Identifiers

PMID28056965
PMCPMC5217455
OpenAlexW2568577132

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.