ArticleJournal of the American Chemical Society2017
Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer-Template Complexes.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Overcoming nucleotide bias in the nonenzymatic copying of RNA templates.Nucleic acids research · 2024Article
- Insight into the structures of unusual base pairs in RNA complexes containing a primer/template/adenosine ligand.RSC chemical biology · 2023Article
- Thermodynamics and kinetics of DNA and RNA dinucleotide hybridization to gaps and overhangs.Biophysical journal · 2023Article
- Direct monitoring of the thermodynamics and kinetics of DNA and RNA dinucleotide dehybridization from gaps and overhangs.bioRxiv : the preprint server for biology · 2023Article
- 2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.Chembiochem : a European journal of chemical biology · 2020Article
- Geochemical influences on nonenzymatic oligomerization of prebiotically relevant cyclic nucleotides.RNA (New York, N.Y.) · 2020Article
- Progress in synthesizing protocells.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension.Biochemistry · 2019Article
- Inosine, but none of the 8-oxo-purines, is a plausible component of a primordial version of RNA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Eighty routes to a ribonucleotide world; dispersion and stringency in the decisive selection.RNA (New York, N.Y.) · 2018Article
- Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide.Journal of the American Chemical Society · 2018Article
- Synthesis of a Nonhydrolyzable Nucleotide Phosphoroimidazolide Analogue That Catalyzes Nonenzymatic RNA Primer Extension.Journal of the American Chemical Society · 2018Article
- Enzyme-free genetic copying of DNA and RNA sequences.Beilstein journal of organic chemistry · 2018Review
- A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5'-phosphoro-2-aminoimidazolide.Biochemistry · 2017Article
- Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Unusual Base-Pairing Interactions in Monomer-Template Complexes.ACS central science · 2016Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Origins of life hypotheses often invoke a transitional phase of nonenzymatic template-directed RNA replication prior to the emergence of ribozyme-catalyzed copying of genetic information. Here, using NMR and ITC, we interrogate the binding affinity of guanosine 5'-monophosphate (GMP) for primer-template complexes when either another GMP, or a helper oligonucleotide, can bind downstream. Binding of GMP to a primer-template complex cannot be significantly enhanced by the possibility of downstream monomer binding, because the affinity of the downstream monomer is weaker than that of the first monomer. Strikingly, GMP binding affinity can be enhanced by ca. 2 orders of magnitude when a helper oligonucleotide is stably bound downstream of the monomer binding site. We compare these thermodynamic parameters to those previously reported for T7 RNA polymerase-mediated replication to help address questions of binding affinity in related nonenzymatic processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.