ArticleInvestigative ophthalmology & visual science2017
Evaluation of Therapeutic Tissue Crosslinking (TXL) for Myopia Using Second Harmonic Generation Signal Microscopy in Rabbit Sclera.
Article in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Polarization-Sensitive Optical Coherence Tomography Imaging of Posterior Staphyloma Edges in Eyes With High Myopia.Investigative ophthalmology & visual science · 2025Observational
- Effects of Genipin Crosslinking of Porcine Perilimbal Sclera on Mechanical Properties and Intraocular Pressure.Bioengineering (Basel, Switzerland) · 2024Article
- On the quantitative analysis of lamellar collagen arrangement with second-harmonic generation imaging.Biomedical optics express · 2024Article
- Article
- A review of the epithelial and stromal effects of corneal collagen crosslinking.The ocular surface · 2023Review
- Scleral growth stunting via sub-Tenon injection of cross-linking solutions in live rabbits.The British journal of ophthalmology · 2023Article
- Age-related enhanced degeneration of bioprosthetic valves due to leaflet calcification, tissue crosslinking, and structural changes.Cardiovascular research · 2023Article
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Changes in Rat Scleral Collagen Structure Induced by UVA-Riboflavin Crosslinking at Various Tissue Depths in Whole Globe Versus Scleral Patch.Translational vision science & technology · 2022Article
- Article
- Collagen Structural Changes in Rat Tarsus After Crosslinking.Translational vision science & technology · 2021Article
- Shaping Eyeballs by Scleral Collagen Cross-Linking: A Hypothesis for Myopia Treatment.Frontiers in medicine · 2021Article
- Changes in Collagen Structure and Permeability of Rat and Human Sclera After Crosslinking.Translational vision science & technology · 2020Article
- Topical Corneal Cross-Linking Solution Delivered Via Corneal Reservoir in Dutch-Belted Rabbits.Translational vision science & technology · 2020Article
- Scleral structure and biomechanics.Progress in retinal and eye research · 2020Review
- Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging.Scientific reports · 2019Article
- Collagen fiber crimping following in vivo UVA-induced corneal crosslinking.Experimental eye research · 2018Article
- Crimp around the globe; patterns of collagen crimp across the corneoscleral shell.Experimental eye research · 2018Article
- Spatial Patterns and Age-Related Changes of the Collagen Crimp in the Human Cornea and Sclera.Investigative ophthalmology & visual science · 2018Article
- Experimental myopia increases and scleral crosslinking using genipin inhibits cyclic softening in the tree shrew sclera.Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) · 2018Article
Corrections and comments
- Erratum issuedErratum.2017
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Purpose: Second harmonic generation signals (SHG) are emitted preferentially from collagenous tissue structures and have been used to evaluate photochemically-induced (CXL) crosslinking changes in the cornea. Since therapeutic tissue crosslinking (TXL) using sodium hydroxymethylglycinate (SMG) of the sclera is a potential treatment for high myopia, we explored the use of SHG microscopy to evaluate the effects. Methods: Single sub-Tenon's (sT) injections (400 μL) using SMG (40-400 mM) were made at the equatorial 12 o'clock position of the right eye of cadaveric rabbit heads (n = 16 pairs). After 3.5 hours, confocal microscopy (CM) was performed using 860 nm two-photon excitation and 400 to 450 nm emission. Pixel density and fiber bundle "waviness" analyses were performed on the images. Crosslinking effects were confirmed using thermal denaturation (Tm) temperature. Comparison experiments with riboflavin photochemical crosslinking were done. Results: Therapeutic tissue crosslinking localization studies indicated that crosslinking changes occurred at the site of injection and in adjacent sectors. Second harmonic generation signals revealed large fibrous collagenous bundled structures that displayed various degrees of waviness. Histogram analysis showed a nearly 6-fold signal increase in 400 mM SMG over 40 mM. This corresponded to a ΔTm = 13°C for 400 mM versus ΔTm = 4°C for 40 mM. Waviness analysis indicated increased fiber straightening as a result of SMG CXL. Conclusions: Second harmonic generation signal intensity and fiber bundle waviness is altered by scleral tissue crosslinking using SMG. These changes provide insights into the macromolecular changes that are induced by therapeutic crosslinking technology and may provide a method to evaluate connective tissue protein changes induced by scleral crosslinking therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.