ArticleCellular microbiology2017
The StcE metalloprotease of enterohaemorrhagic Escherichia coli reduces the inner mucus layer and promotes adherence to human colonic epithelium ex vivo.
Article in Cellular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 82 citations in OpenAlex.
- Substrate secretion by different EHEC secretion systems during their interaction with epithelial cells.Virulence · 2026Article
- Restoring O-glycosylation and expression of MUC2 limits progression of colorectal cancer.Cell death & disease · 2026Article
- The VaginalMicroorganisms · 2026Article
- Bacterial zinc metalloproteases as facilitators of pathogenic success: a review.Archives of microbiology · 2026Review
- CanineMicrobial genomics · 2026Article
- Mucinase fromFrontiers in microbiology · 2026Article
- Article
- Vat-Mediated Mucus Penetration Enables Genotoxic Activity ofInternational journal of molecular sciences · 2025Article
- Enterohemorrhagic E. coli (EHEC) and the microbiome.PLoS pathogens · 2025Article
- Mucins and Their Roles in Asthma.Immunological reviews · 2025Review
- Article
- Cultivating complexity: Advancements in establishing in vitro models for the mucus-adhering gut microbiota.Microbial biotechnology · 2024Review
- FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155.Veterinary research · 2024Article
- Differential Colonization and Mucus Ultrastructure Visualization in Bovine Ileal and Rectal Organoid-Derived Monolayers Exposed to EnterohemorrhagicInternational journal of molecular sciences · 2024Article
- Stepwise assembly and release of Tc toxins from Yersinia entomophaga.Nature microbiology · 2024Article
- Identification of a genomic cluster related to hypersecretion of intestinal mucus and mucinolytic activity of atypical enteropathogenicFrontiers in cellular and infection microbiology · 2024Article
- A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins.Nature chemical biology · 2023Article
- A mucin-regulated adhesin determines the spatial organization and inflammatory character of a bacterial symbiont in the vertebrate gut.Cell host & microbe · 2023Article
- Genomic Characterization ofAntibiotics (Basel, Switzerland) · 2023Article
- O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.Essays in biochemistry · 2023Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) is a major foodborne pathogen and tightly adheres to human colonic epithelium by forming attaching/effacing lesions. To reach the epithelial surface, EHEC must penetrate the thick mucus layer protecting the colonic epithelium. In this study, we investigated how EHEC interacts with the intestinal mucus layer using mucin-producing LS174T colon carcinoma cells and human colonic mucosal biopsies. The level of EHEC binding and attaching/effacing lesion formation in LS174T cells was higher compared to mucin-deficient colon carcinoma cell lines, and initial adherence was independent of the presence of flagellin, Escherichia coli common pilus, or long polar fimbriae. Although EHEC infection did not affect gene expression of secreted mucins, it resulted in reduced MUC2 glycoprotein levels. This effect was dependent on the catalytic activity of the secreted metalloprotease StcE, which reduced the inner mucus layer and thereby promoted EHEC access and binding to the epithelium in vitro and ex vivo. Given the lack of efficient therapies against EHEC infection, StcE may represent a suitable target for future treatment and prevention strategies.
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Registered trials
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