ArticlePlant methods2017
Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 337 citations in OpenAlex.
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- Sequence dynamics and plastome evolution: decoding the complete chloroplast genome of Oenothera drummondii and comparative analysis within Oenothera (Onagraceae).Functional & integrative genomics · 2025Article
- A simplified low-cost and reliable plant genomic DNA extraction method for PCR-based genotyping and screening.Plant methods · 2025Article
- LAYLA: development of a comprehensive and cross-sample program for detecting structural variants and its application to citrus cultivars.Breeding science · 2025Article
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- Genetic Trends in General Combining Ability for Maize Yield-Related Traits in Northeast China.Current issues in molecular biology · 2025Article
- Genome-wide association study for plant height and ear height in maize under well-watered and water-stressed conditions.BMC genomics · 2025Article
- Ionic liquid-assisted seed genomic DNA extraction for advanced sequencing applications.Plant methods · 2025Article
- Improved genomic DNA extraction from citrus species using a modified CTAB method.Molecular biology reports · 2025Article
- SSR-based molecular characterization of Verticillium wilt resistance in Iranian cotton cultivars.Biochemistry and biophysics reports · 2025Article
- A novel approach for DNA extraction of white spot syndrome virus detection in penaeid shrimp.Scientific reports · 2025Article
- First report on isolation and characterization of Bacillus Sp. associated with honey bee brood disease.Scientific reports · 2025Article
17 more citing papers are in PubMed but not listed here.
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2 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe world's top three cereals, based on their monetary value, are rice, wheat, and corn. In cereal crops, DNA extraction is difficult owing to rigid non-cellulose components in the cell wall of leaves and high starch and protein content in grains. The advanced techniques in molecular biology require pure and quick extraction of DNA. The majority of existing DNA extraction methods rely on long incubation and multiple precipitations or commercially available kits to produce contaminant-free high molecular weight DNA.
resultsIn this study, we compared three different methods used for the isolation of high-quality genomic DNA from the grains of cereal crop,
conclusionThe modified Mericon extraction method was found to be the most efficient DNA extraction method, capable to provide high DNA yields with better quality, affordable cost and less time.
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