ArticleScientific reports2017
Functional analysis of the ABCs of eye color in Helicoverpa armigera with CRISPR/Cas9-induced mutations.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 130 citations in OpenAlex.
- Functional Analysis of theInsects · 2025Article
- CRISPR/Cas9-Mediated Knockout of the White Gene inInternational journal of molecular sciences · 2025Article
- SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs.Insect biochemistry and molecular biology · 2025Article
- Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentata.Scientific reports · 2025Article
- The white gene as a transgenesis marker for the cricket Gryllus bimaculatus.G3 (Bethesda, Md.) · 2024Article
- CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Biomolecules · 2024Review
- Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control inInsects · 2023Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus.Scientific reports · 2023Article
- 8-HQA adjusts the number and diversity of bacteria in the gut microbiome ofFrontiers in microbiology · 2023Article
- Advancements and prospects of CRISPR/Cas9 technologies for abiotic and biotic stresses in sugar beet.Frontiers in genetics · 2023Review
- Insect opsins and evo-devo: what have we learned in 25 years?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Melanin Synthesis Pathway Interruption: CRISPR/Cas9-mediated Knockout of dopa decarboxylase (DDC) in Harmonia axyridis (Coleoptera: Coccinellidae).Journal of insect science (Online) · 2022Article
- Functional Diversity of the Lepidopteran ATP-Binding Cassette Transporters.Journal of molecular evolution · 2022Review
- RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.).Scientific reports · 2022Article
- Atypical strategies for cuticle pigmentation in the blood-feeding hemipteran Rhodnius prolixus.Genetics · 2022Article
- CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug.Scientific reports · 2022Article
- Cas9-mediated gene editing in the black-legged tick,iScience · 2022Article
- Article
- Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many insect pigments are localized in subcellular pigment granules, and transport of pigment precursors from the cytoplasm is accomplished by ABC proteins. Drosophila melanogaster has three half-transporter genes (white, scarlet, and brown, all affecting eye pigments) and Bombyx mori has a fourth (ok). The White, Brown, Scarlet and Ok proteins each have one transmembrane and one cytoplasmic domain and they heterodimerize to form functional transporters with different substrate specificities. We used CRISPR/Cas9 to create somatic and germ-line knockout mutations of these four genes in the noctuid moth Helicoverpa armigera. Somatic knockouts of white block pigmentation of the egg, first instar larva and adult eye, but germ-line knockouts of white are recessive lethal in the embryo. Knockouts of scarlet are viable and produce pigmentless first instar larvae and yellow adult eyes lacking xanthommatin. Knockouts of brown show no phenotypic effects on viability or pigmentation. Knockouts of ok are viable and produce translucent larval cuticle and black eyes. CRISPR/Cas9-induced mutations are a useful tool for analyzing how essential and non-essential genes interact to produce the diversity of insect pigmentation patterns found in nature.
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