ArticleScientific reports2017
Simultaneous high-speed imaging and optogenetic inhibition in the intact mouse brain.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Article
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Multicolor two-photon light-patterning microscope exploiting the spatio-temporal properties of a fiber bundle.Biomedical optics express · 2024Article
- Functional imaging through scattering medium via fluorescence speckle demixing and localization.Optics express · 2023Article
- Functional imaging through scattering medium via fluorescence speckle demixing and localization.ArXiv · 2023Article
- Scanless two-photon voltage imaging.Research square · 2023Article
- Computational optics for high-throughput imaging of neural activity.Neurophotonics · 2022Review
- Advances in computer-generated holography for targeted neuronal modulation.Neurophotonics · 2022Review
- Implantable acousto-optic window for monitoring ultrasound-mediated neuromodulationNeurophotonics · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins.eLife · 2021Article
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Scanless two-photon excitation with temporal focusing.Nature methods · 2020Review
- Azimuthal multiplexing 3D diffractive optics.Scientific reports · 2020Article
- High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning.Cell reports · 2020Article
- Chronic Cranial Windows for Long Term Multimodal Neurovascular Imaging in Mice.Frontiers in physiology · 2020Article
- 3D Localization for Light-Field Microscopy via Convolutional Sparse Coding on Epipolar Images.IEEE transactions on computational imaging · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
Genetically encoded calcium indicators and optogenetic actuators can report and manipulate the activity of specific neuronal populations. However, applying imaging and optogenetics simultaneously has been difficult to establish in the mammalian brain, even though combining the techniques would provide a powerful approach to reveal the functional organization of neural circuits. Here, we developed a technique based on patterned two-photon illumination to allow fast scanless imaging of GCaMP6 signals in the intact mouse brain at the same time as single-photon optogenetic inhibition with Archaerhodopsin. Using combined imaging and electrophysiological recording, we demonstrate that single and short bursts of action potentials in pyramidal neurons can be detected in the scanless modality at acquisition frequencies up to 1 kHz. Moreover, we demonstrate that our system strongly reduces the artifacts in the fluorescence detection that are induced by single-photon optogenetic illumination. Finally, we validated our technique investigating the role of parvalbumin-positive (PV) interneurons in the control of spontaneous cortical dynamics. Monitoring the activity of cellular populations on a precise spatiotemporal scale while manipulating neuronal activity with optogenetics provides a powerful tool to causally elucidate the cellular mechanisms underlying circuit function in the intact mammalian brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.