ArticleProceedings of the National Academy of Sciences of the United States of America2017
Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Clicking viruses-with chemistry toward mechanisms in infection.Journal of virology · 2025Review
- Pathogenicity of La Jolla Virus inViruses · 2022Article
- Review
- Virion structure andJournal of virology · 2021Article
- Organization of the Structural Protein Region of La Jolla Virus Isolated from the Invasive Pest InsectViruses · 2021Article
- pH-Induced Conformational Changes of Human Bocavirus Capsids.Journal of virology · 2021Article
- Capsid opening enables genome release of iflaviruses.Science advances · 2021Article
- Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy.Human gene therapy · 2019Article
- The atomic structures of shrimp nodaviruses reveal new dimeric spike structures and particle polymorphism.Communications biology · 2019Article
- Virion structure and genome delivery mechanism of sacbrood honeybee virus.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid.Journal of virology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses from the family Iflaviridae are insect pathogens. Many of them, including slow bee paralysis virus (SBPV), cause lethal diseases in honeybees and bumblebees, resulting in agricultural losses. Iflaviruses have nonenveloped icosahedral virions containing single-stranded RNA genomes. However, their genome release mechanism is unknown. Here, we show that low pH promotes SBPV genome release, indicating that the virus may use endosomes to enter host cells. We used cryo-EM to study a heterogeneous population of SBPV virions at pH 5.5. We determined the structures of SBPV particles before and after genome release to resolutions of 3.3 and 3.4 Å, respectively. The capsids of SBPV virions in low pH are not expanded. Thus, SBPV does not appear to form "altered" particles with pores in their capsids before genome release, as is the case in many related picornaviruses. The egress of the genome from SBPV virions is associated with a loss of interpentamer contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the capsid. Pores that are 7 Å in diameter form around icosahedral threefold symmetry axes. We speculate that they serve as channels for the genome release. Our findings provide an atomic-level characterization of the genome release mechanism of iflaviruses.
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