Evidence map›Paper›PMID 28053217›Full record

ArticleJournal of clinical microbiology2017

High Interlaboratory Reproducibility and Accuracy of Next-Generation-Sequencing-Based Bacterial Genotyping in a Ring Trial.

Alexander Mellmann, Paal Skytt Andersen, Stefan Bletz, Alexander W Friedrich, Thomas A Kohl, Berit Lilje, Stefan Niemann, Karola Prior, John W Rossen, Dag Harmsen

Abstract readEvaluation StudyMulticenter Study
In one paragraph

Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Increase ofMicrobiology spectrum · 2025
    Article
  6. Article
  7. Article
  8. Identification ofMicroorganisms · 2024
    Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Pathogens (Basel, Switzerland) · 2021
    Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Alexander MellmannInstitute of Hygiene, University Hospital Münster, Münster, Germany mellmann@uni-muenster.de.ORCID 0000-0002-0649-5185
Paal Skytt AndersenDepartment of Microbiology and Infection Control, Statens Serum Institut (SSI), Copenhagen, Denmark.
Stefan BletzInstitute of Hygiene, University Hospital Münster, Münster, Germany.
Alexander W FriedrichDepartment of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Thomas A KohlMolecular and Experimental Mycobacteriology, Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Borstel, Germany.
Berit LiljeDepartment of Microbiology and Infection Control, Statens Serum Institut (SSI), Copenhagen, Denmark.
Stefan NiemannMolecular and Experimental Mycobacteriology, Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Borstel, Germany.
Karola PriorDepartment of Periodontology and Restorative Dentistry, University Hospital Münster, Münster, Germany.
John W RossenDepartment of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Dag HarmsenDepartment of Periodontology and Restorative Dentistry, University Hospital Münster, Münster, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Today, next-generation whole-genome sequencing (WGS) is increasingly used to determine the genetic relationships of bacteria on a nearly whole-genome level for infection control purposes and molecular surveillance. Here, we conducted a multicenter ring trial comprising five laboratories to determine the reproducibility and accuracy of WGS-based typing. The participating laboratories sequenced 20 blind-coded

Indexed as

Genome, BacterialGenotyping TechniquesHumansMolecular EpidemiologyMolecular TypingReproducibility of ResultsSequence Analysis, DNAStaphylococcus aureuscgMLSTinterlaboratory reproducibilitymolecular subtypingring trialwhole-genome sequencing

Identifiers

PMID28053217
PMCPMC5328459

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.