ArticleStem cells and development2017
The NOTCH Ligand JAG1 Regulates GDNF Expression in Sertoli Cells.
Article in Stem cells and development, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 68 citations in OpenAlex.
- Role of Estrogen Signaling in Notch Pathway Activation in Sertoli Cells.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Reference-informed evaluation of batch correction for single-cell omics data with overcorrection awareness.Communications biology · 2025Article
- Sertoli cells as a hub in testicular development and male reproductive.Frontiers in cell and developmental biology · 2025Review
- Analysis of histone modifications in key cellular subpopulations in the context of azoospermia using spermatogenic single-cell RNA-seq data.Frontiers in bioinformatics · 2025Article
- Androgen blockage impairs proliferation and function of Sertoli cells via Wee1 and Lfng.Cell communication and signaling : CCS · 2024Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- Comparative Transcriptome Analysis Reveals Sexually Dimorphic Gene Expression in the Gonads ofAnimals : an open access journal from MDPI · 2023Article
- Notch pathway is required for protection against heat stress in spermatogonial stem cells in medaka.Fish physiology and biochemistry · 2023Article
- Article
- Regulation of spermatogonial stem cell self-renewal and proliferation in mammals.Histology and histopathology · 2022Review
- Human obstructive (postvasectomy) and nonobstructive azoospermia - Insights from scRNA-Seq and transcriptome analysis.Genes & diseases · 2022Article
- Transcriptional profiling of β-2MStem cell reports · 2022Article
- Article
- Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.Journal of cell communication and signaling · 2022Article
- Nuclear and Membrane Receptors for Sex Steroids Are Involved in the Regulation of Delta/Serrate/LAG-2 Proteins in Rodent Sertoli Cells.International journal of molecular sciences · 2022Article
- Sertoli Cell-Germ Cell Interactions Within the Niche: Paracrine and Juxtacrine Molecular Communications.Frontiers in endocrinology · 2022Review
- CellCall: integrating paired ligand-receptor and transcription factor activities for cell-cell communication.Nucleic acids research · 2021Article
- Spermatogonial stem cell transplantation into nonablated mouse recipient testes.Stem cell reports · 2021Article
- The involvement of bioactive factors in the self-renewal and stemness maintenance of spermatogonial stem cells.Molecular and cellular biochemistry · 2021Review
- Involvement of Cytokines and Hormones in the Development of Spermatogenesis In Vitro from Spermatogonial Cells of Cyclophosphamide-Treated Immature Mice.International journal of molecular sciences · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In the seminiferous epithelium of the testis, Sertoli cells are key niche cells directing proliferation and differentiation of spermatogonial stem cells (SSCs) into spermatozoa. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), which is essential for SSC self-renewal and progenitor expansion. While the role of GDNF in the testis stem cell niche is established, little is known about how this factor is regulated. Our previous studies on NOTCH activity in Sertoli cells demonstrated a role of this pathway in limiting stem/progenitor cell numbers, thus ultimately downregulating sperm cell output. In this study we demonstrate through a double-mutant mouse model that NOTCH signaling in Sertoli cells functions solely through the canonical pathway. Further, we demonstrate through Dual luciferase assay and chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR) analysis that the NOTCH targets HES1 and HEY1, which are transcriptional repressors, directly downregulate GDNF expression by binding to the Gdnf promoter, thus antagonizing the effects of FSH/cAMP. Finally, we demonstrate that testicular stem/progenitors cells are activating NOTCH signaling in Sertoli cells in vivo and in vitro through the NOTCH ligand JAG1 at their surface, indicating that these cells may ensure their own homeostasis through negative feedback regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.