ArticleScientific reports2017
Validation of suitable normalizers for miR expression patterns analysis covering tumour heterogeneity.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Identification of stably expressed microRNAs in plasma from high-grade serous ovarian carcinoma and benign tumor patients.Molecular biology reports · 2023Article
- Unraveling the Potential of miRNAs from CSCs as an Emerging Clinical Tool for Breast Cancer Diagnosis and Prognosis.International journal of molecular sciences · 2023Review
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- Machine learning and bioinformatics approaches for classification and clinical detection of bevacizumab responsive glioblastoma subtypes based on miRNA expression.Scientific reports · 2022Article
- Targeted MicroRNA Profiling in Gastric Cancer with Clinical Assessement.Balkan journal of medical genetics : BJMG · 2021Article
- Identification of miR-29c-3p as a Robust Normalizer for Urine microRNA Studies in Bladder Cancer.Biomedicines · 2020Article
- BIK drives an aggressive breast cancer phenotype through sublethal apoptosis and predicts poor prognosis of ER-positive breast cancer.Cell death & disease · 2020Article
- Deregulation of cancer-stem-cell-associated miRNAs in tissues and sera of colorectal cancer patients.Oncotarget · 2020Article
- Identification of housekeeping genes for microRNA expression analysis in kidney tissues of Pkd1 deficient mouse models.Scientific reports · 2020Article
- Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer.Journal of biological engineering · 2020Article
- Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples.PloS one · 2020Article
- Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting.Scientific reports · 2019Article
- Peripheral blood microRNA levels in females with cocaine use disorder.Journal of psychiatric research · 2019Article
- Identification and validation of circulating miRNAs as endogenous controls in obstructive sleep apnea.PloS one · 2019Article
- Identification of Stably Expressed Reference microRNAs in Epithelial Ovarian Cancer.In vivo (Athens, Greece)Article
- miRNA Expression in Ovarian Cancer in Fresh Frozen, Formalin-fixed Paraffin-embedded and Plasma Samples.In vivo (Athens, Greece)Article
- Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oncogenic microRNAs (miRs) have emerged as diagnostic biomarkers and novel molecular targets for anti-cancer drug therapies. Real-time quantitative PCR (qPCR) is one of the most powerful techniques for analyzing miRs; however, the use of unsuitable normalizers might bias the results. Tumour heterogeneity makes even more difficult the selection of an adequate endogenous normalizer control. Here, we have evaluated five potential referenced small RNAs (U6, rRNA5s, SNORD44, SNORD24 and hsa-miR-24c-3p) using RedFinder algorisms to perform a stability expression analysis in i) normal colon cells, ii) colon and breast cancer cell lines and iii) cancer stem-like cell subpopulations. We identified SNORD44 as a suitable housekeeping gene for qPCR analysis comparing normal and cancer cells. However, this small nucleolar RNA was not a useful normalizer for cancer stem-like cell subpopulations versus subpopulations without stemness properties. In addition, we show for the first time that hsa-miR-24c-3p is the most stable normalizer for comparing these two subpopulations. Also, we have identified by bioinformatic and qPCR analysis, different miR expression patterns in colon cancer versus non tumour cells using the previously selected suitable normalizers. Our results emphasize the importance of select suitable normalizers to ensure the robustness and reliability of qPCR data for analyzing miR expression.
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