ArticleScientific reports2017
Sequence specific sorting of DNA molecules with FACS using 3dPCR.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Accessible, robust ultrahigh-throughput microbiome analysis via integration of uniform droplet-templated emulsification and FACS.PloS one · 2026Article
- Continuous FACS Sorting of Double Emulsion Picoreactors with a 3D-Printed Vertical Mixer.Analytical chemistry · 2025Article
- Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.bioRxiv : the preprint server for biology · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics.Nature protocols · 2024Review
- NOVAsort for error-free droplet microfluidics.Nature communications · 2024Article
- DEPICT-seq: Single-Cell Transcriptomic Analysis of Rare Cell Subsets Isolated via Nucleic Acid Cytometry.Analytical chemistry · 2024Article
- Flow Cytometry: The Next Revolution.Cells · 2023Review
- Flow cytometric printing of double emulsions into open droplet arrays.Lab on a chip · 2023Article
- Double Emulsion Flow Cytometry for Rapid Single Genome Detection.Methods in molecular biology (Clifton, N.J.) · 2023Article
- FIDELITY: A quality control system for droplet microfluidics.Science advances · 2022Article
- Systematic characterization of effect of flow rates and buffer compositions on double emulsion droplet volumes and stability.Lab on a chip · 2022Article
- Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.Biotechnology journal · 2022Article
- Sequencing Ultrarare Targets with Compound Nucleic Acid Cytometry.Analytical chemistry · 2021Article
- An asymmetric flow-focusing droplet generator promotes rapid mixing of reagents.Scientific reports · 2021Article
- Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS.Analytical chemistry · 2020Article
- Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery.Lab on a chip · 2020Article
- Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery.Nucleic acids research · 2020Article
- Precision public health to inhibit the contagion of disease and move toward a future in which microbes spread health.BMC infectious diseases · 2019Review
- Particle-Templated Emulsification for Microfluidics-Free Digital Biology.Analytical chemistry · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Genetic heterogeneity is an important feature of many biological systems, but introduces technical challenges to their characterization. Even with the best modern instruments, only a small fraction of DNA molecules present in a sample can be read, and they are recovered in the form of short, hundred-base reads. In this paper, we introduce 3dPCR, a method to sort DNA molecules with sequence specificity. 3dPCR allows heterogeneous populations of DNA to be sorted to recover long targets for deep sequencing. It is valuable whenever a target sequence is rare in a mixed population, such as for characterizing mutations in heterogeneous cancer cell populations or identifying cells containing a specific genetic sequence or infected with a target virus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.