ArticleJournal of the American Society for Mass Spectrometry2017
Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System.
Article in Journal of the American Society for Mass Spectrometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Rapid Cold Fixation of Mouse Colon (Col'RFix) Enables High-Resolution Mass Spectrometry Imaging.Analytical chemistry · 2026Article
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- (Pre)Clinical Metabolomics Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI.Journal of the American Society for Mass Spectrometry · 2024Article
- Mass Spectrometry Imaging of Biomaterials.Materials (Basel, Switzerland) · 2023Review
- Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs).Journal of the American Society for Mass Spectrometry · 2023Article
- Single-cell and spatial multi-omics in the plant sciences: Technical advances, applications, and perspectives.Plant communications · 2023Review
- Chemical imaging reveals diverse functions of tricarboxylic acid metabolites in root growth and development.Nature communications · 2023Article
- Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non-flat samples.Journal of mass spectrometry : JMS · 2023Article
- Spatial metabolomics reveals skeletal myofiber subtypes.Science advances · 2023Article
- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Biological insights from multi-omics analysis strategies: Complex pleotropic effects associated with autophagy.Frontiers in plant science · 2023Article
- Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View.International journal of molecular sciences · 2022Review
- Mass Spectrometry Imaging for Spatial Chemical Profiling of Vegetative Parts of Plants.Plants (Basel, Switzerland) · 2022Review
- Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging.International journal of molecular sciences · 2021Review
- Storing and Reading Information in Mixtures of Fluorescent Molecules.ACS central science · 2021Article
- The utility of metabolomics as a tool to inform maize biology.Plant communications · 2021Review
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Single-Cell Metabolomics by Mass Spectrometry Imaging.Advances in experimental medicine and biology · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between ~4, ~7, and ~45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders. Graphical Abstract ᅟ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.