ArticlemBio2017
Proximity Interactions among Basal Body Components in Trypanosoma brucei Identify Novel Regulators of Basal Body Biogenesis and Inheritance.
Article in mBio, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- SF3B1 phosphorylation is an evolutionarily conserved step in spliceosome activation carried out by the divergent, OTS964-insensitive kinase CRK9 in trypanosomes.Nucleic acids research · 2026Article
- Discovery of effectors for casein kinase signaling in the African trypanosome.Scientific reports · 2025Article
- ARL3 GTPases facilitate ODA16 unloading from IFT in motile cilia.Science advances · 2024Article
- Identification of 30 transition fibre proteins in Trypanosoma brucei reveals a complex and dynamic structure.Journal of cell science · 2024Article
- Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography.Life science alliance · 2024Article
- Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared.The Journal of biological chemistry · 2023Article
- Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defects.Nature communications · 2022Article
- Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.Molecular & cellular proteomics : MCP · 2022Review
- Tag Thy Neighbour: Nanometre-Scale Insights Into Kinetoplastid Parasites With Proximity Dependent Biotinylation.Frontiers in cellular and infection microbiology · 2022Review
- Intrabody-Induced Cell Death by Targeting the T. brucei Cytoskeletal ProteinMicrobiology spectrum · 2021Article
- APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei.mSphere · 2021Article
- The kinetoplastid-specific phosphatase KPP1 attenuates PLK activity to facilitate flagellum inheritance inScience signaling · 2021Article
- In vivo interactome profiling by enzyme-catalyzed proximity labeling.Cell & bioscience · 2021Review
- CEP164C regulates flagellum length in stable flagella.The Journal of cell biology · 2021Article
- Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome.PloS one · 2021Article
- A cytoskeletal protein complex is essential for division of intracellular amastigotes ofThe Journal of biological chemistry · 2020Article
- Maintenance of hook complex integrity and centrin arm assembly facilitates flagellum inheritance inThe Journal of biological chemistry · 2020Article
- FAZ27 cooperates with FLAM3 and ClpGM6 to maintain cell morphology inJournal of cell science · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
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7 authors.
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Abstract
The basal body shares similar architecture with centrioles in animals and is involved in nucleating flagellar axonemal microtubules in flagellated eukaryotes. The early-branching Trypanosoma brucei possesses a motile flagellum nucleated from the basal body that consists of a mature basal body and an adjacent pro-basal body. Little is known about the basal body proteome and its roles in basal body biogenesis and flagellar axoneme assembly in T. brucei Here, we report the identification of 14 conserved centriole/basal body protein homologs and 25 trypanosome-specific basal body proteins. These proteins localize to distinct subdomains of the basal body, and several of them form a ring-like structure surrounding the basal body barrel. Functional characterization of representative basal body proteins revealed distinct roles in basal body duplication/separation and flagellar axoneme assembly. Overall, this work identified novel proteins required for basal body duplication and separation and uncovered new functions of conserved basal body proteins in basal body duplication and separation, highlighting an unusual mechanism of basal body biogenesis and inheritance in this early divergent eukaryote. IMPORTANCE: The basal body in the early-branching protozoan Trypanosoma brucei nucleates flagellum assembly and also regulates organelle segregation, cell morphogenesis, and cell division. However, the molecular composition and the assembly process of the basal body remain poorly understood. Here, we identify 14 conserved basal body proteins and 25 trypanosome-specific basal body proteins via bioinformatics, localization-based screening, and proximity-dependent biotin identification. We further localized these proteins to distinct subdomains of the basal body by using fluorescence microscopy and superresolution microscopy, discovered novel regulators of basal body duplication and separation, and uncovered new functions of conserved basal body proteins in basal body duplication and separation. This work lays the foundation for dissecting the mechanisms underlying basal body biogenesis and inheritance in T. brucei.
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