ArticlePLoS pathogens2017
An Oxygen-Sensing Two-Component System in the Burkholderia cepacia Complex Regulates Biofilm, Intracellular Invasion, and Pathogenicity.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Functional analysis ofMicrobiology spectrum · 2026Article
- The IrlS2-IrlR2 two-component system is a global regulator of biofilm formation, stress adaptation, and virulence inmSphere · 2026Article
- Adaptation of Burkholderia cenocepacia to low oxygen drives changes consistent with adaptation to chronic infection.BMC microbiology · 2026Article
- De novo mutations mediate phenotypic switching in an opportunistic human lung pathogen.Nature communications · 2025Article
- Transcriptional Regulatory Systems in Pseudomonas: A Comparative Analysis of Helix-Turn-Helix Domains and Two-Component Signal Transduction Networks.International journal of molecular sciences · 2025Article
- Harnessing hypoxia: bacterial adaptation and chronic infection in cystic fibrosis.FEMS microbiology reviews · 2025Review
- The mntH gene of Burkholderia cenocepacia influences motility and quorum sensing to control virulence.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Genotype-by-environment interactions govern fitness changes associated with adaptive mutations in two-component response systems.Frontiers in genetics · 2024Article
- Detecting bacterial adaptation within individual microbiomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Identification of Key Factors for Anoxic Survival ofInternational journal of molecular sciences · 2022Article
- Article
- Proteomic Response ofLife (Basel, Switzerland) · 2021Article
- Bacterial evolution during human infection: Adapt and live or adapt and die.PLoS pathogens · 2021Review
- Evolution towards Virulence in amBio · 2021Article
- RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence.PLoS pathogens · 2021Article
- Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells.Scientific reports · 2021Article
- Time-course transcriptome analysis reveals the mechanisms of Burkholderia sp. adaptation to high phenol concentrations.Applied microbiology and biotechnology · 2020Article
- Regulation of Virulence by Two-Component Systems in PathogenicInfection and immunity · 2020Review
- Burkholderia cepacia Complex Contact-Dependent Growth Inhibition Systems Mediate Interbacterial Competition.Journal of bacteriology · 2019Article
- The response of Sphingopyxis granuli strain TFA to the hostile anoxic condition.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia dolosa is a member of the Burkholderia cepacia complex (BCC), which is a group of bacteria that cause chronic lung infection in patients with cystic fibrosis (CF) and can be associated with outbreaks carrying high morbidity and mortality. While investigating the genomic diversity of B. dolosa strains collected from an outbreak among CF patients, we previously identified fixL as a gene showing signs of strong positive selection. This gene has homology to fixL of the rhizobial FixL/FixJ two-component system. The goals of this study were to determine the functions of FixLJ and their role in virulence in B. dolosa. We generated a fixLJ deletion mutant and complemented controls in B. dolosa strain AU0158. Using a fixK-lacZ reporter we found that FixLJ was activated in low oxygen in multiple BCC species. In a murine pneumonia model, the B. dolosa fixLJ deletion mutant was cleared faster from the lungs and spleen than wild-type B. dolosa strain AU0158 at 7 days post infection. Interestingly, the fixLJ deletion mutant made more biofilm, albeit with altered structure, but was less motile than strain AU0158. Using RNA-seq with in vitro grown bacteria, we found ~11% of the genome was differentially expressed in the fixLJ deletion mutant relative to strain AU0158. Multiple flagella-associated genes were down-regulated in the fixLJ deletion mutant, so we also evaluated virulence of a fliC deletion mutant, which lacks a flagellum. We saw no difference in the ability of the fliC deletion mutant to persist in the murine model relative to strain AU0158, suggesting factors other than flagella caused the phenotype of decreased persistence. We found the fixLJ deletion mutant to be less invasive in human lung epithelial and macrophage-like cells. In conclusion, B. dolosa fixLJ is a global regulator that controls biofilm formation, motility, intracellular invasion/persistence, and virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.