ArticlePloS one2017
A Low-Level Carbon Dioxide Laser Promotes Fibroblast Proliferation and Migration through Activation of Akt, ERK, and JNK.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effectiveness of low-level laser therapy in reducing pain score and healing time of recurrent aphthous stomatitis: a systematic review and meta-analysis.Systematic reviews · 2024Pooled it
- Optimisation of low-level light therapy (lllt) parameters and evaluation of its immunomodulatory effect on fibroblasts.Lasers in medical science · 2026Article
- Intense pulsed light rejuvenates UVB-induced photoaging in human keratinocytes and guinea pig skin by inhibition of ERK-AP-1-MMP pathway.Scientific reports · 2026Article
- Effects of photobiomodulation on human dental pulp stem cells treated with a bone xenograft: An in vitro study.Lasers in medical science · 2026Article
- Activation of Cannabinoid Receptor 1 Enhances Wound Healing by Promoting the Proliferative Phase.International journal of molecular sciences · 2026Article
- Effects of Photobiomodulation on Osteoarthritis from In Vivo and In Vitro Studies: A Narrative Review.International journal of molecular sciences · 2025Review
- Early Intervention With an Ultrapulse COJournal of cosmetic dermatology · 2025Article
- Laser Photobiomodulation: What Are the Ideal Parameters for Each Type of Laser Used in Dental Practice to Promote Fibroblast Proliferation and Differentiation? A Systematic Review.Life (Basel, Switzerland) · 2025Review
- In Vitro Effects of Low-Level Laser Therapy on the Viability and Secretory Activity of Resting Macrophages.Biomedicines · 2025Article
- Effects of low-level laser irradiation on canine fibroblasts.The Journal of veterinary medical science · 2025Article
- Assessing the Long-Term Effectiveness of Fractional COJournal of clinical medicine · 2025Article
- Effects of Low-Level Gallium-Aluminum-Arsenide Laser Therapy on Human Dermal Fibroblast Proliferation, ATP, and ROS Levels.Journal of lasers in medical sciences · 2025Article
- Use of carbon dioxide fractional laser in the management of lower limb ischemia in patients with diabetes: a case report : COThe Journal of international medical research · 2024Article
- The Effects and Mechanisms of PBM Therapy in Accelerating Orthodontic Tooth Movement.Biomolecules · 2023Review
- Suppression of matrix degradation and amelioration of disc degeneration by a 970-nm diode laser via inhibition of the p38 MAPK pathway in a rabbit model.Lasers in medical science · 2023Article
- Regulatory Processes of the Canonical Wnt/β-Catenin Pathway and Photobiomodulation in Diabetic Wound Repair.International journal of molecular sciences · 2022Review
- Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing GOncology letters · 2022Article
- The Effect of Low-Level Laser Therapy on the Viability of Human Dental Pulp Stem Cells.Journal of lasers in medical sciences · 2022Article
- Cellular Signalling and Photobiomodulation in Chronic Wound Repair.International journal of molecular sciences · 2021Review
- Cyclometalated Ru(II) β-carboline complexes induce cell cycle arrest and apoptosis in human HeLa cervical cancer cells via suppressing ERK and Akt signaling.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLow-level laser therapy (LLLT) with various types of lasers promotes fibroblast proliferation and migration during the process of wound healing. Although LLLT with a carbon dioxide (CO2) laser was also reported to promote wound healing, the underlying mechanisms at the cellular level have not been previously described. Herein, we investigated the effect of LLLT with a CO2 laser on fibroblast proliferation and migration. MATERIALS AND
methodsCultured human dermal fibroblasts were prepared. MTS and cell migration assays were performed with fibroblasts after LLLT with a CO2 laser at various doses (0.1, 0.5, 1.0, 2.0, or 5.0 J/cm2) to observe the effects of LLLT with a CO2 laser on the proliferation and migration of fibroblasts. The non-irradiated group served as the control. Moreover, western blot analysis was performed using fibroblasts after LLLT with a CO2 laser to analyze changes in the activities of Akt, extracellular signal-regulated kinase (ERK), and Jun N-terminal kinase (JNK), which are signaling molecules associated with cell proliferation and migration. Finally, the MTS assay, a cell migration assay, and western blot analysis were performed using fibroblasts treated with inhibitors of Akt, ERK, or JNK before LLLT with a CO2 laser.
resultsIn MTS and cell migration assays, fibroblast proliferation and migration were promoted after LLLT with a CO2 laser at 1.0 J/cm2. Western blot analysis revealed that Akt, ERK, and JNK activities were promoted in fibroblasts after LLLT with a CO2 laser at 1.0 J/cm2. Moreover, inhibition of Akt, ERK, or JNK significantly blocked fibroblast proliferation and migration.
conclusionsThese findings suggested that LLLT with a CO2 laser would accelerate wound healing by promoting the proliferation and migration of fibroblasts. Activation of Akt, ERK, and JNK was essential for CO2 laser-induced proliferation and migration of fibroblasts.
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