Evidence map›Paper›PMID 28045587›Full record

ArticleBioengineered2017

Conjugation of ϕBT1-derived integrative plasmid pDZL802 in Amycolatopsis mediterranei U32.

Chen Li, Li Zhou, Ying Wang, Guoping Zhao, Xiaoming Ding

Open access · goldAbstract read
In one paragraph

Article in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. CRISPR-Cas12a-Assisted Genome Editing inFrontiers in bioengineering and biotechnology · 2020
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Chen Lia State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering , School of Life Sciences, Fudan University , Shanghai , China.ORCID http://orcid.org/0000-0001-8757-1153
Li Zhoua State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering , School of Life Sciences, Fudan University , Shanghai , China.
Ying Wanga State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering , School of Life Sciences, Fudan University , Shanghai , China.ORCID http://orcid.org/0000-0002-5841-3321
Guoping Zhaoa State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering , School of Life Sciences, Fudan University , Shanghai , China.
Xiaoming Dinga State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering , School of Life Sciences, Fudan University , Shanghai , China.
Fudan University · CNChinese University of Hong Kong · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that use this genus have been developed. Amycolatopsis mediterranei is an industrial-scale producer of the important antibiotic rifamycin, which plays a vital role in antimycobacterial therapy. Genetic studies of Amycolatopsis species have progressed slowly due to the lack of efficient transformation methods and stable plasmid vectors. In A. mediterranei U32, electroporation and replicable plasmid vectors have been developed. Here, we establish a simple and efficient conjugal system by transferring integrative plasmid pDZL802 from ET12567 (pUZ8002) to A. mediterranei U32, with an efficiency of 4 × 10

Indexed as

ActinobacteriaConjugation, GeneticDNA, BacterialPlasmidsRifamycinsTransfectionTransformation, GeneticDNA, BacterialRifamycinsAmycolatopsisconjugal transfer systemintegrative plasmidmetabolic engineeringnatural products

Identifiers

PMID28045587
PMCPMC5639847
OpenAlexW2561646648

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.