ArticleBiochemistry2017
Anaerobic Heme Degradation: ChuY Is an Anaerobilin Reductase That Exhibits Kinetic Cooperativity.
Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Biochemical and functional characterization ofFrontiers in microbiology · 2026Article
- Heme in Bacterial Pathogenesis and as an Antimicrobial Target.Chemical reviews · 2025Review
- Exploring heme and iron acquisition strategies of Porphyromonas gingivalis-current facts and hypotheses.FEMS microbiology reviews · 2025Review
- The phylogenomic landscape of extended-spectrum β-lactamase producing Citrobacter species isolated from surface water.BMC genomics · 2023Article
- A new member of the flavodoxin superfamily from Fusobacterium nucleatum that functions in heme trafficking and reduction of anaerobilin.The Journal of biological chemistry · 2023Article
- Experimental Evolution Reveals a Novel Ene Reductase That Detoxifies α,β-Unsaturated Aldehydes in Listeria monocytogenes.Microbiology spectrum · 2023Article
- Elucidating the origins of phycocyanobilin biosynthesis and phycobiliproteins.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Evidence for Porphyrin-Mediated Electron Transfer in the Radical SAM Enzyme HutW.Biochemistry · 2023Article
- The impact of iron and heme availability on the healthy human gut microbiome in vivo and in vitro.Cell chemical biology · 2023Article
- Noncanonical Functions of Enzyme Cofactors as Building Blocks in Natural Product Biosynthesis.JACS Au · 2022Review
- Innovation and tinkering in the evolution of oxidases.Protein science : a publication of the Protein Society · 2022Review
- Class C Radical SAM Methyltransferases Involved in Anaerobic Heme Degradation.ACS bio & med chem Au · 2022Review
- The Small RNA CyaR Activates Translation of the Outer Membrane Haem ReceptorFrontiers in microbiology · 2022Article
- Making and breaking carbon-carbon bonds in class C radical SAM methyltransferases.Journal of inorganic biochemistry · 2022Review
- Iron Transport and Metabolism in Escherichia, Shigella, and Salmonella.EcoSal Plus · 2021Article
- HutW fromBiochemistry · 2021Article
- New Insight into the Mechanism of Anaerobic Heme Degradation.Biochemistry · 2019Article
- HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida.Veterinary research · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Heme catabolism is an important biochemical process that many bacterial pathogens utilize to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further processing for transport out of the cell or conversion to another useful cofactor. In previous work, we presented in vitro evidence of an anaerobic heme degradation pathway in Escherichia coli O157:H7. Consistent with reactions that have been reported for other radical S-adenosyl-l-methionine methyltransferases, ChuW transfers a methyl group to heme by a radical-mediated mechanism and catalyzes the β-scission of the porphyrin macrocycle. This facilitates iron release and the production of a new linear tetrapyrrole termed "anaerobilin". In this work, we describe the structure and function of ChuY, an enzyme expressed downstream from chuW within the same heme utilization operon. ChuY is structurally similar to biliverdin reductase and forms a dimeric complex in solution that reduces anaerobilin to the product we have termed anaerorubin. Steady state analysis of ChuY exhibits kinetic cooperativity that is best explained by a random addition mechanism with a kinetically preferred path for initial reduced nicotinamide adenine dinucleotide phosphate binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.