ArticleACS chemical neuroscience2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight.
Article in ACS chemical neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Modulating Chromophore Flexibility in GEVIs through Threonine-Based Molecular Switches Reveals an Influence of Membrane Curvature on Protein Activity.ACS sensors · 2025Article
- From Plankton to Primates: How VSP Sequence Diversity Shapes Voltage Sensing.International journal of molecular sciences · 2025Article
- In vivo Pirt-Marina voltage sensor imaging detects primary sensory neuron-specific voltage dynamics and neuronal plasticity changes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Positive-going hybrid indicators for voltage imaging in excitable cells and tissues.Science advances · 2025Article
- Imaging sensory transmission and neuronal plasticity in primary sensory neurons with a positively tuned voltage indicator.Nature communications · 2025Article
- Stretch-induced endogenous electric fields drive directed collective cell migration in vivo.Nature materials · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.Scientific reports · 2024Article
- Exploring the potential of water channels for developing genetically encoded reporters and biosensors for diffusion-weighted MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents.bioRxiv : the preprint server for biology · 2024Article
- A positively tuned voltage indicator for extended electrical recordings in the brain.Nature methods · 2023Article
- High-speed low-light in vivo two-photon voltage imaging of large neuronal populations.Nature methods · 2023Article
- Designed active-site library reveals thousands of functional GFP variants.Nature communications · 2023Article
- Review
- Simultaneous Measurement of Changes in Mitochondrial and Endoplasmic Reticulum Free Calcium in Pancreatic Beta Cells.Biosensors · 2023Article
- Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types.Science (New York, N.Y.) · 2022Article
- Review
- Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.Cell · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Genetically encoded calcium indicators (GECIs) produce unprecedentedly large signals that have enabled routine optical recording of single neuron activity in vivo in rodent brain. Genetically encoded voltage indicators (GEVIs) offer a more direct measure of neuronal electrical status, however the signal-to-noise characteristics and signal polarity of the probes developed to date have precluded routine use in vivo. We applied directed evolution to target modulable areas of the fluorescent protein in GEVI ArcLight to create the first GFP-based GEVI (Marina) that exhibits a ΔF/ΔV with a positive slope relationship. We found that only three rounds of site-directed mutagenesis produced a family of "brightening" GEVIs with voltage sensitivities comparable to that seen in the parent probe ArcLight. This shift in signal polarity is an essential first step to producing voltage indicators with signal-to-noise characteristics comparable to GECIs to support widespread use in vivo.
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