ArticleScientific reports2017
Cerebrospinal fluid lens-free microscopy: a new tool for the laboratory diagnosis of meningitis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Small-volume detection: platform developments for clinically-relevant applications.Journal of nanobiotechnology · 2021Review
- High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging.Sensors (Basel, Switzerland) · 2021Article
- A narrative review of central nervous system involvement in acute leukemias.Annals of translational medicine · 2021Review
- Lensless, reflection-based dark-field microscopy (RDFM) on a CMOS chip.Biomedical optics express · 2020Article
- Point-of-care multiplexed diagnosis of meningitis using the FilmArray® ME panel technology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- 3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging.Scientific reports · 2019Article
- Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture.Journal of visualized experiments : JoVE · 2018Article
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Authors and funding
7 authors.
Funding
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Abstract
Cerebrospinal fluid cytology is performed by operator-dependant light microscopy as part of the routine laboratory work-flow diagnosis of meningitis. We evaluated operator-independent lens-free microscopy numeration of erythrocytes and leukocytes for the cytological diagnosis of meningitis. In a first step, prospective optical microscopy counts of leukocytes done by five different operators yielded an overall 16.7% misclassification of 72 cerebrospinal fluid specimens in meningitis/non-meningitis categories using a 10 leukocyte/μL cut-off. In a second step, the lens-free microscopy algorithm adapted for counting cerebrospinal fluid cells and discriminating leukocytes from erythrocytes was modified step-by-step in the prospective analysis of 215 cerebrospinal fluid specimens. The definite algorithm yielded a 100% sensitivity and a 86% specificity compared to confirmed diagnostics. In a third step, a blind lens-free microscopic analysis of 116 cerebrospinal fluid specimens, including six cases of microbiology-confirmed infectious meningitis, yielded a 100% sensitivity and a 79% specificity. Adapted lens-free microscopy is thus emerging as an operator-independent technique for the rapid numeration of leukocytes and erythrocytes in cerebrospinal fluid. In particular, this technique is well suited to the rapid diagnosis of meningitis at point-of-care laboratories.
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