Evidence map›Paper›PMID 28045071›Full record

ArticleScientific reports2017

Magnetic Resonance Microscopy (MRM) of Single Mammalian Myofibers and Myonuclei.

Choong H Lee, Niclas Bengtsson, Stephen M Chrzanowski, Jeremy J Flint, Glenn A Walter, Stephen J Blackband

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Experimental demonstration of diffusion limitations on resolution and SNR in MR microscopy.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2023
    Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 1 country.

Choong H LeeCenter for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY, 10012, USA.
Niclas BengtssonChamberlain Laboratory, University of Washington, Department of Neurology, Seattle, Washington, 98195, USA.
Stephen M ChrzanowskiDepartment of Physiology and Functional Genomics, University of Florida College of Medicine, Gainesville, FL, 32610-0274, USA.
Jeremy J FlintDepartment of Neuroscience, University of Florida College of Medicine, Gainesville, FL, 32611, USA.
Glenn A WalterNational High Magnetic Field Laboratory, Tallahassee, FL, 32310, USA.
Stephen J BlackbandDepartment of Neuroscience, University of Florida College of Medicine, Gainesville, FL, 32611, USA.
University of Florida · USFlorida College · USNational High Magnetic Field Laboratory · USNew York University · USUniversity of Washington · US

Funding

Multidisciplinary Training Program in HypertensionT32HL083810 · NHLBI · UNIVERSITY OF FLORIDA · PI WOOD, CHARLES E · 2007 to 2017
$2.5M
Development of MR Microscopy at the Cellular LevelR01EB012874 · NIBIB · UNIVERSITY OF FLORIDA · PI BLACKBAND, STEPHEN J. · 2010 to 2013
$2.1M
Console upgrade for microimaging and solid state NMR spectroscopy at 600 MHzS10RR031637 · NCRR · UNIVERSITY OF FLORIDA · PI LONG, JOANNA R · 2011 to 2011
$450k
NCRR NIH HHS S10 RR031637NHLBI NIH HHS T32 HL083810NIBIB NIH HHS R01 EB012874
6 · The paper itself

Abstract

Recently, the first magnetic resonance microscopy (MRM) images at the cellular level in isolated mammalian brain tissues were obtained using microsurface coils. These methods can elucidate the cellular origins of MR signals and describe how these signals change over the course of disease progression and therapy. In this work, we explore the capability of these microimaging techniques to visualize mouse muscle fibers and their nuclei. Isolated myofibers expressing lacZ were imaged with and without a stain for β-galactosidase activity (S-Gal + ferric ammonium citrate) that produces both optical and MR contrast. We found that MRM can be used to image single myofibers with 6-μm resolution. The ability to image single myofibers will serve as a valuable tool to study MR properties attributed to healthy and myopathic cells. The ability to image nuclei tagged with MR/Optical gene markers may also find wide use in cell lineage MRI studies.

Indexed as

AnimalsGenes, ReporterImaging, Three-DimensionalMagnetic Resonance ImagingMice, Inbred C57BLMicroscopyMicroscopy, InterferenceMuscle Fibers, Skeletal

Identifiers

PMID28045071
PMCPMC5206738
OpenAlexW2564667958

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.