ArticleChemical science2016
A protein-dye hybrid system as a narrow range tunable intracellular pH sensor.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- pH-Assisted multichannel heat shock monitoring in the endoplasmic reticulum with a pyridinium fluorophore.Chemical science · 2024Article
- Ratiometric, pH-Sensitive Probe for Monitoring siRNA Delivery.Journal of the American Chemical Society · 2023Article
- A reversible plasmonic nanoprobe for dynamic imaging of intracellular pH during endocytosis.Chemical science · 2022Article
- Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.Molecules (Basel, Switzerland) · 2021Review
- A new pentacyclic pyrylium fluorescent probe that responds to pH imbalance during apoptosis.Chemical science · 2020Article
- Cell organelle targeting of near-infrared croconaine dye controls photothermal outcome.Chemical communications (Cambridge, England) · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate monitoring of pH variations inside cells is important for the early diagnosis of diseases such as cancer. Even though a variety of different pH sensors are available, construction of a custom-made sensor array for measuring minute variations in a narrow biological pH window, using easily available constituents, is a challenge. Here we report two-component hybrid sensors derived from a protein and organic dye nanoparticles whose sensitivity range can be tuned by choosing different ratios of the components, to monitor the minute pH variations in a given system. The dye interacts noncovalently with the protein at lower pH and covalently at higher pH, triggering two distinguishable fluorescent signals at 700 and 480 nm, respectively. The pH sensitivity region of the probe can be tuned for every unit of the pH window resulting in custom-made pH sensors. These narrow range tunable pH sensors have been used to monitor pH variations in HeLa cells using the fluorescence imaging technique.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.