ArticleChemical science2016
Precision targeted ruthenium(ii) luminophores; highly effective probes for cell imaging by stimulated emission depletion (STED) microscopy.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 87 citations in OpenAlex.
- Canonical Ru(ii) tris-polypyridyl complexes as confocal-compatible stains for lignified secondary-wall domains inRSC advances · 2026Article
- Advances in pH-Sensitive Luminescent Transition Metal Complexes for Theranostic Applications.Chemistry, an Asian journal · 2026Review
- Ruthenium Complexes in Protein Science: Heading a New Generation of Therapeutic and Analytical Tools.Angewandte Chemie (International ed. in English) · 2026Review
- Transition metal complexes as optical probes for super-resolution microscopy.Nature reviews. Chemistry · 2025Review
- Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications.Chemical reviews · 2024Review
- Unprecedented enantio-selective live-cell mitochondrial DNA super-resolution imaging and photo-sensitizing by the chiral ruthenium polypyridyl DNA "light-switch".Nucleic acids research · 2023Article
- Reversibly Tuning Electrochemiluminescence with Stimulated Emission Route for Single-Cell Imaging.Research (Washington, D.C.) · 2023Article
- Luminescent Metal Complexes as Emerging Tools for Lipid Imaging.Topics in current chemistry (Cham) · 2022Review
- Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing.Topics in current chemistry (Cham) · 2022Review
- A Biophysical Study of Ru(II) Polypyridyl Complex, Properties and its Interaction with DNA.Journal of fluorescence · 2022Article
- Metalloimmunotherapy with Rhodium and Ruthenium Complexes: Targeting Tumor-Associated Macrophages.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Ru(ii)/BODIPY core co-encapsulated ratiometric nanotools for intracellular ORSC chemical biology · 2021Article
- Strategies to promote permeation and vectorization, and reduce cytotoxicity of metal complex luminophores for bioimaging and intracellular sensing.RSC chemical biology · 2021Review
- Os(II)-Bridged Polyarginine Conjugates: The Additive Effects of Peptides in Promoting or Preventing Permeation in Cells and Multicellular Tumor Spheroids.Inorganic chemistry · 2021Article
- Emerging Molecular Receptors for the Specific-Target Delivery of Ruthenium and Gold Complexes into Cancer Cells.Molecules (Basel, Switzerland) · 2021Review
- Phosphorescent metal complexes as theranostic anticancer agents: combining imaging and therapy in a single molecule.Chemical science · 2021Review
- Photophysics and Cell Uptake of Self-Assembled Ru(II)Polypyridyl Vesicles.Frontiers in chemistry · 2020Article
- A simple empirical algorithm for optimising depletion power and resolution for dye and system specific STED imaging.Journal of microscopy · 2019Article
- Dual-targeted peptide-conjugated multifunctional fluorescent probe with AIEgen for efficient nucleus-specific imaging and long-term tracing of cancer cells.Chemical science · 2017Article
- Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells.Chemical science · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy has undergone a dramatic evolution over the past two decades with development of super-resolution far-field microscopy methods that break the light diffraction limited resolution of conventional microscopy, offering unprecedented opportunity to interrogate cellular processes at the nanoscale. However, these methods make special demands of the luminescent agents used for contrast and development of probes suited to super-resolution fluorescent methods is still relatively in its infancy. In spite of their many photophysical advantages, metal complex luminophores have not yet been considered as probes in this regard, where to date, only organic fluorophores have been applied. Here, we report the first examples of metal complex luminophores applied as probes for use in stimulated emission depletion (STED) microscopy. Exemplified with endoplasmic reticulum and nuclear targeting complexes we demonstrate that luminescent Ru(ii) polypyridyl complexes can, through signal peptide targeting, be precisely and selectively delivered to key cell organelles without the need for membrane permeabilization, to give high quality STED images of these organelles. Detailed features of the tubular ER structure are revealed and in the case of the nuclear targeting probe we exploit the molecular light switch properties of a dipyrido[3,2-
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.