ArticleJournal of virological methods2017
PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts.
Article in Journal of virological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Leveraging machine learning and big data techniques to map the global patent landscape of phage therapy.Nature biotechnology · 2024Article
- The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater.Environmental science & technology · 2023Article
- Inference of the Life Cycle of Environmental Phages from Genomic Signature Distances to Their Hosts.Viruses · 2023Article
- Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.Biotechnology journal · 2022Article
- Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics.Cell reports methods · 2022Article
- Targeted Single-Cell RNA and DNA Sequencing With Fluorescence-Activated Droplet Merger.Analytical chemistry · 2020Article
- Review
- Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.Lab on a chip · 2017Review
- Efficient extraction of oil from droplet microfluidic emulsions.Biomicrofluidics · 2017Article
- A universal primer-independent next-generation sequencing approach for investigations of norovirus outbreaks and novel variants.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Characterizing virus-host relationships is critical for understanding the impact of a virus on an ecosystem, but is challenging with existing techniques, particularly for uncultivable species. We present a general, cultivation-free approach for identifying phage-associated bacterial cells. Using PCR-activated cell sorting, we interrogate millions of individual bacteria for the presence of specific phage nucleic acids. If the nucleic acids are present, the bacteria are recovered via sorting and their genomes analyzed. This allows targeted recovery of all possible host species in a diverse population associated with a specific phage, and can be easily targeted to identify the hosts of different phages by modifying the PCR primers used for detection. Moreover, this technique allows quantification of free phage particles, as benchmarked against the "gold standard" of virus enumeration, the plaque assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.