ArticleDevelopmental cell2017
Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics.
Article in Developmental cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
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- The Fruit Fly Auxodrome: a computer vision setup for longitudinal studies of Drosophila development.Genetics · 2026Article
- Behavioral age-detection in individuals reconstructs minute-scale developmental transcriptomics.bioRxiv : the preprint server for biology · 2025Article
- HarnessingSensors (Basel, Switzerland) · 2025Review
- CED-3 caspase promotes dismantling but not onset of non-apoptotic linker cell death inbioRxiv : the preprint server for biology · 2025Article
- Automated Platforms inMicromachines · 2025Review
- Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology.Lab on a chip · 2025Article
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Automated dual olfactory device for studying head/tail chemosensation inAPL bioengineering · 2024Article
- Worm-Based Diagnosis Combining Microfluidics toward Early Cancer Screening.Micromachines · 2024Review
- A circadian-like gene network programs the timing and dosage of heterochronic miRNA transcription during C. elegans development.Developmental cell · 2023Article
- High-resolution imaging and analysis of the intestinal bacterial load ofRSC advances · 2023Article
- Inferring a spatial code of cell-cell interactions across a whole animal body.PLoS computational biology · 2022Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Focal adhesion-mediated cell anchoring and migration: from in vitro to in vivo.Development (Cambridge, England) · 2022Article
- Deviations from temporal scaling support a stage-specific regulation for C. elegans postembryonic development.BMC biology · 2022Article
- SALSA, a genetically encoded biosensor for spatiotemporal quantification of Notch signal transduction in vivo.Developmental cell · 2022Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Long-term studies of Caenorhabditis elegans larval development traditionally require tedious manual observations because larvae must move to develop, and existing immobilization techniques either perturb development or are unsuited for young larvae. Here, we present a simple microfluidic device to simultaneously follow development of ten C. elegans larvae at high spatiotemporal resolution from hatching to adulthood (∼3 days). Animals grown in microchambers are periodically immobilized by compression to allow high-quality imaging of even weak fluorescence signals. Using the device, we obtain cell-cycle statistics for C. elegans vulval development, a paradigm for organogenesis. We combine Nomarski and multichannel fluorescence microscopy to study processes such as cell-fate specification, cell death, and transdifferentiation throughout post-embryonic development. Finally, we generate time-lapse movies of complex neural arborization through automated image registration. Our technique opens the door to quantitative analysis of time-dependent phenomena governing cellular behavior during C. elegans larval development.
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Registered trials
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