ArticleImmunology2017
Isolation and high-dimensional phenotyping of gastrointestinal immune cells.
Article in Immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory Challenge.Methods and protocols · 2026Article
- Dissecting immunophenotypic diversity in multiple myeloma via mass cytometry: a call for harmonized gating strategies.Frontiers in immunology · 2025Review
- Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune Cells.Methods and protocols · 2022Article
- Susceptibility to Infections During Acute Liver Injury Depends on Transient Disruption of Liver Macrophage Niche.Frontiers in immunology · 2022Article
- CyTOFFrontiers in immunology · 2022Review
- PD-L1Nature communications · 2021Article
- MicroRNA-146a limits tumorigenic inflammation in colorectal cancer.Nature communications · 2021Article
- Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD.JHEP reports : innovation in hepatology · 2020Article
- Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity.Cell reports · 2019Article
- CLA-supplemented diet accelerates experimental colorectal cancer by inducing TGF-β-producing macrophages and T cells.Mucosal immunology · 2019Article
- Acute microglia ablation induces neurodegeneration in the somatosensory system.Nature communications · 2018Article
- Article
- IL-33 signalling in liver immune cells enhances drug-induced liver injury and inflammation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gastrointestinal immune system plays a pivotal role in the host relationship with food antigens, the homeostatic microbiome and enteric pathogens. Here, we describe how to collect and process liver and intestinal samples to efficiently isolate and analyse resident immune cells. Furthermore, we describe a step-by-step methodology showing how to high-dimensionally immunophenotype resident leucocytes using cytometry by time-of-flight, providing a well-characterized antibody platform that allows the identification of every leucocyte subset simultaneously. This protocol also includes instructions to purify and cultivate primary murine hepatocytes, a powerful tool to assess basic cell biology and toxicology assays. Gut and liver samples from the same mouse can be collected, processed and stained in less than 6 hr. This protocol enables the recovery of several populations of purified and viable immune cells from solid and fibrous organs, preventing unwanted loss of adherent cells during isolation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.