Evidence map›Paper›PMID 28039620›Full record

ArticleIn vitro cellular & developmental biology. Animal2017

Primordial germ cell differentiation of nuclear transfer embryonic stem cells using surface modified electroconductive scaffolds.

Tarlan Eslami-Arshaghi, Saeid Vakilian, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi, Masoud Soleimani, Mohammad Salehi

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Tarlan Eslami-ArshaghiStem Cells Technology Research Center, Tehran, Iran.
Saeid VakilianStem Cells Technology Research Center, Tehran, Iran.
Ehsan SeyedjafariDepartment of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Abdolreza ArdeshirylajimiStem Cells Technology Research Center, Tehran, Iran.
Masoud SoleimaniDepartment of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mohammad SalehiCellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. M.Salehi@sbmu.ac.ir.
Stem Cell Technology Research Center · IRShahid Beheshti University of Medical Sciences · IRTarbiat Modares University · IRUniversity of Tehran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A combination of nanotopographical cues and surface modification of collagen and fibronectin is a potential platform in primordial germ cells (PGCs) differentiation. In the present study, the synergistic effect of nanotopography and surface modification on differentiation of nuclear transfer embryonic stem cells (nt-ESCs) toward PGC lineage was investigated. In order to achieve this goal, poly-anyline (PANi) was mix within poly-L-lactic acid (PLLA). Afterward, the random composite mats were fabricated using PLLA and PANi mix solution. The nanofiber topography notably upregulated the expressions of prdm14, mvh and c-kit compared with tissue culture polystyrene (TCP). Moreover, the combination of nanofiber topography and surface modification resulted in more enhancement of PGCs differentiation compared with non-modified nanofibrous scaffold. Additionally, gene expression results showed that mvh and c-kit were expressed at higher intensity in cells exposed to collagen and fibronectin rather than collagen or fibronectin solitary. These results demonstrated the importance of combined effect of collagen and fibronectin in order to develop a functional extracellular matrix (ECM) mimic in directing stem cell fate and the potential of such biofunctional scaffolds for treatment of infertility.

Indexed as

Cell DifferentiationElectric ConductivityNuclear Transfer TechniquesAniline CompoundsAnimalsCell ProliferationCell ShapeCell SurvivalEmbryonic Stem CellsGene Expression RegulationGerm CellsMiceNanofibersPolyestersReal-Time Polymerase Chain ReactionRNA, MessengerAniline CompoundspolyanilinePolyesterspoly(lactide)RNA, MessengerCollagenElectroconductive scaffoldsFibronectinNuclear transfer embryonic stem cellsPrimordial germ cells

Identifiers

PMID28039620
OpenAlexW2560888122

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.