ArticleNucleic acids research2017
Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 12 citations in OpenAlex.
- A short history of Auto-Rickshaw.Acta crystallographica. Section D, Structural biology · 2026Review
- A bacterial defense system targeting modified cytosine of phage genomic DNA.Nature communications · 2026Article
- Crystal structures of monomeric BsmI restriction endonuclease reveal coordinated sequential cleavage of two DNA strands.Communications biology · 2025Article
- Restriction endonuclease T.Smu451I with new cleavage specificity-neoschizomer of T.AsuI.Folia microbiologica · 2021Article
- The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence.Nucleic acids research · 2021Article
- Predicting Hot Spot Residues at Protein-DNA Binding Interfaces Based on Sequence Information.Interdisciplinary sciences, computational life sciences · 2021Article
- Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation.Nucleic acids research · 2020Article
- Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate.Nature communications · 2020Article
- Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family.Nucleic acids research · 2019Article
- Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI.Nucleic acids research · 2019Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although all Type II restriction endonucleases catalyze phosphodiester bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies ranging from monomers, dimers, tetramers to higher order oligomers to generate a double strand break in DNA. Type IIP restriction endonuclease AgeI recognizes a palindromic sequence 5΄-A/CCGGT-3΄ and cuts it ('/' denotes the cleavage site) producing staggered DNA ends. Here, we present crystal structures of AgeI in apo and DNA-bound forms. The structure of AgeI is similar to the restriction enzymes that share in their target sites a conserved CCGG tetranucleotide and a cleavage pattern. Structure analysis and biochemical data indicate, that AgeI is a monomer in the apo-form both in the crystal and in solution, however, it binds and cleaves the palindromic target site as a dimer. DNA cleavage mechanism of AgeI is novel among Type IIP restriction endonucleases.
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