ArticleStem cell research & therapy2016
Comparison of a teratogenic transcriptome-based predictive test based on human embryonic versus inducible pluripotent stem cells.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- In vitro models of valproic acid to assess neurodevelopmental toxicity: A scoping review.Epilepsia · 2025Article
- Investigating the applicability domain of the hiPSC-based PluriLum assay: an embryotoxicity assessment of chemicals and drugs.Archives of toxicology · 2024Article
- Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells.Cell death discovery · 2023Article
- A New Strategy for the Old Challenge of Thalidomide: Systems Biology Prioritization of Potential Immunomodulatory Drug (IMiD)-Targeted Transcription Factors.International journal of molecular sciences · 2023Article
- Developmental Toxicity Studies: The Path towards Humanized 3D Stem Cell-Based Models.International journal of molecular sciences · 2023Review
- Optimization of the TeraTox Assay for Preclinical Teratogenicity Assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- A review of possible therapies for multiple sclerosis.Molecular and cellular biochemistry · 2021Review
- Chromatin Imbalance as the Vertex Between Fetal Valproate Syndrome and Chromatinopathies.Frontiers in cell and developmental biology · 2021Review
- A novel human pluripotent stem cell-based assay to predict developmental toxicity.Archives of toxicology · 2020Article
- Testing of female reproductive disorders.Archives of toxicology · 2020Article
- The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods.Archives of toxicology · 2020Article
- Review
- Reproductive toxicity of boron.EXCLI journal · 2020Article
- Review
- Strategies to retain properties of bone marrow-derived mesenchymal stem cells ex vivo.Annals of the New York Academy of Sciences · 2017Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman embryonic stem cells (hESCs) partially recapitulate early embryonic three germ layer development, allowing testing of potential teratogenic hazards. Because use of hESCs is ethically debated, we investigated the potential for human induced pluripotent stem cells (hiPSCs) to replace hESCs in such tests.
methodsThree cell lines, comprising hiPSCs (foreskin and IMR90) and hESCs (H9) were differentiated for 14 days. Their transcriptome profiles were obtained on day 0 and day 14 and analyzed by comprehensive bioinformatics tools.
resultsThe transcriptomes on day 14 showed that more than 70% of the "developmental genes" (regulated genes with > 2-fold change on day 14 compared to day 0) exhibited variability among cell lines. The developmental genes belonging to all three cell lines captured biological processes and KEGG pathways related to all three germ layer embryonic development. In addition, transcriptome profiles were obtained after 14 days of exposure to teratogenic valproic acid (VPA) during differentiation. Although the differentially regulated genes between treated and untreated samples showed more than 90% variability among cell lines, VPA clearly antagonized the expression of developmental genes in all cell lines: suppressing upregulated developmental genes, while inducing downregulated ones. To quantify VPA-disturbed development based on developmental genes, we estimated the "developmental potency" (D
conclusionsDespite differences in genes deregulated by VPA, uniform D
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