ArticlePloS one2016
The Change of a Medically Important Genus: Worldwide Occurrence of Genetically Diverse Novel Brucella Species in Exotic Frogs.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- First report of aFrontiers in veterinary science · 2026Article
- A new member of the novel, non-core Brucella clade: An exotic frog isolate closely related to atypical Brucella isolates from recent human brucellosis cases in Australia.BMC microbiology · 2025Article
- Global spillover of land-derived microbes to Ocean hosts: Sources, transmission pathways, and one health threats.Environmental science and ecotechnology · 2025Review
- Investigation of genomic island 2 deciphers the evolution of a genus Brucella.Scientific reports · 2025Article
- Brucella microti and Rodent-Borne Brucellosis: A Neglected Public Health Threat.Zoonoses and public health · 2025Review
- Review
- Isolation ofFrontiers in microbiology · 2023Article
- Core Genome Multilocus Sequence Typing Scheme for Improved Characterization and Epidemiological Surveillance of Pathogenic Brucella.Journal of clinical microbiology · 2022Article
- The Retrospective on AtypicalMicroorganisms · 2022Review
- High-Resolution Melting PCR as Rapid Genotyping Tool forMicroorganisms · 2022Article
- Atypical Brucella inopinata-Like Species in 2 Marine Toads.Emerging infectious diseases · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Editorial: Pathogenomics of the GenusFrontiers in microbiology · 2021Article
- Bovine brucellosis - a comprehensive review.The veterinary quarterly · 2021Review
- Review
- Expanding the host range: infection of a reptilian host (Furcifer pardalis) by an atypical Brucella strain.Antonie van Leeuwenhoek · 2020Article
- Identification and Characterization of the First Virulent Phages, Including a Novel Jumbo Virus, InfectingInternational journal of molecular sciences · 2020Article
- Omp2b Porin Alteration in the Course of Evolution ofFrontiers in microbiology · 2020Article
- Application of Whole Genome Sequencing and Pan-Family Multi-Locus Sequence Analysis to Characterize Relationships Within the FamilyFrontiers in microbiology · 2020Article
- Spinal arthritis in invasive cane toads is linked to rate of dispersal as well as to latitude.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genus Brucella comprises various species of both veterinary and human medical importance. All species are genetically highly related to each other, sharing intra-species average nucleotide identities (ANI) of > 99%. Infections occur among various warm-blooded animal species, marine mammals, and humans. Until recently, amphibians had not been recognized as a host for Brucella. In this study, however, we show that novel Brucella species are distributed among exotic frogs worldwide. Comparative recA gene analysis of 36 frog isolates from various continents and different frog species revealed an unexpected high genetic diversity, not observed among classical Brucella species. In phylogenetic reconstructions the isolates consequently formed various clusters and grouped together with atypical more distantly related brucellae, like B. inopinata, strain BO2, and Australian isolates from rodents, some of which were isolated as human pathogens. Of one frog isolate (10RB9215) the genome sequence was determined. Comparative genome analysis of this isolate and the classical Brucella species revealed additional genetic material, absent from classical Brucella species but present in Ochrobactrum, the closest genetic neighbor of Brucella, and in other soil associated genera of the Alphaproteobacteria. The presence of gene clusters encoding for additional metabolic functions, flanked by tRNAs and mobile genetic elements, as well as by bacteriophages is suggestive for a different ecology compared to classical Brucella species. Furthermore it suggests that amphibian isolates may represent a link between free living soil saprophytes and the pathogenic Brucella with a preferred intracellular habitat. We therefore assume that brucellae from frogs have a reservoir in soil and, in contrast to classical brucellae, undergo extensive horizontal gene transfer.
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