ArticlePlant methods2016
A method for detecting single mRNA molecules in
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 81 citations in OpenAlex.
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- Advances in single-cell and spatial multi-omics for plant development and stress responses.Plant diversity · 2026Review
- Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections.Plant communications · 2026Article
- Spatial transcriptomics reveals expression gradients in developing wheat inflorescences at cellular resolution.The Plant cell · 2026Article
- Long Noncoding RNAs as Emerging Regulators of Seed Development, Germination, and Senescence.International journal of molecular sciences · 2025Review
- Ribosome stalling-induced NIP5;1 mRNA decay triggers ARGONAUTE1-dependent transcription downregulation.Nucleic acids research · 2025Article
- Spatial metatranscriptomics resolves host-bacteria-fungi interactomes.Nature biotechnology · 2024Article
- Frontiers in plant RNA research in ICAR2023: from lab to innovative agriculture.Plant molecular biology · 2024Article
- A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes.The Plant cell · 2024Article
- Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.Nature communications · 2024Article
- RNA and Protein Detection by Single-Molecule Fluorescent in Situ Hybridization (smFISH) Combined with Immunofluorescence in the Budding Yeast S. cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol.Plant methods · 2023Article
- Article
- Visualizing and counting single RNA molecules in intact plant tissues.Nature plants · 2023Article
- Whole-mount smFISH allows combining RNA and protein quantification at cellular and subcellular resolution.Nature plants · 2023Article
- Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization.Journal of experimental botany · 2023Article
- Promoter-pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt.The EMBO journal · 2023Article
- A 3D gene expression atlas of the floral meristem based on spatial reconstruction of single nucleus RNA sequencing data.Nature communications · 2022Article
- Localizing Total mRNA in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Number of necessary training examples for Neural Networks with different number of trainable parameters.Journal of pathology informatics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDespite advances in other model organisms, there are currently no techniques to explore cell-to-cell variation and sub-cellular localization of RNA molecules at the single-cell level in plants.
resultsHere we describe a method for imaging individual mRNA molecules in
conclusionThis method allows single molecule RNA in situ to be exploited as a powerful tool for studying gene regulation in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.