ArticleMolecular biology of the cell2017
Model-guided optogenetic study of PKA signaling in budding yeast.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Global impact on metabolic capacity of yeast cell factories by optogenetic control of the cAMP-PKA axis.Applied and environmental microbiology · 2026Article
- Single-cell analysis and control of microbial systems using optogenetics.Current opinion in microbiology · 2026Review
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- The Yeast Optogenetic Toolkit (yOTK) for Spatiotemporal Control of Gene Expression in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Performing in spite of starvation: How Saccharomyces cerevisiae maintains robust growth when facing famine zones in industrial bioreactors.Microbial biotechnology · 2023Article
- Analysis of localized cAMP perturbations within a tissue reveal the effects of a local, dynamic gap junction state on ERK signaling.PLoS computational biology · 2022Article
- Genetic Screening ofFrontiers in microbiology · 2022Article
- Modeling and analysis of the macronutrient signaling network in budding yeast.Molecular biology of the cell · 2021Article
- A yeast FRET biosensor enlightens cAMP signaling.Molecular biology of the cell · 2021Article
- Cell-machine interfaces for characterizing gene regulatory network dynamics.Current opinion in systems biology · 2019Article
- The finite state projection based Fisher information matrix approach to estimate information and optimize single-cell experiments.PLoS computational biology · 2019Article
- Extended Robust Boolean Network of Budding Yeast Cell Cycle.Journal of medical signals and sensorsArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In eukaryotes, protein kinase A (PKA) is a master regulator of cell proliferation and survival. The activity of PKA is subject to elaborate control and exhibits complex time dynamics. To probe the quantitative attributes of PKA dynamics in the yeast Saccharomyces cerevisiae, we developed an optogenetic strategy that uses a photoactivatable adenylate cyclase to achieve real-time regulation of cAMP and the PKA pathway. We capitalize on the precise and rapid control afforded by this optogenetic tool, together with quantitative computational modeling, to study the properties of feedback in the PKA signaling network and dissect the nonintuitive dynamic effects that ensue from perturbing its components. Our analyses reveal that negative feedback channeled through the Ras1/2 GTPase is delayed, pinpointing its time scale and its contribution to the dynamic features of the cAMP/PKA signaling network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.