ArticleACS synthetic biology2017
A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering.
Article in ACS synthetic biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- High throughput screening of eukaryotic release factor 1 variants to enhance noncanonical amino acid incorporation.bioRxiv : the preprint server for biology · 2025Article
- Establishment of High-Throughput Screening Protocol Based on Isomerase UsingJournal of microbiology and biotechnology · 2025Article
- Identifying and Engineering Flavin Dependent Halogenases for Selective Biocatalysis.Accounts of chemical research · 2024Article
- Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators.Cell reports methods · 2023Article
- A primer to directed evolution: current methodologies and future directions.RSC chemical biology · 2023Review
- Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals.Cell systems · 2022Article
- Assembly and Evolution of Artificial Metalloenzymes withinJournal of the American Chemical Society · 2022Article
- Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Directed Evolution Methods for Enzyme Engineering.Molecules (Basel, Switzerland) · 2021Review
- Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.Protein engineering, design & selection : PEDS · 2021Article
- Multicomponent Bioluminescence Imaging with a π-Extended Luciferin.Journal of the American Chemical Society · 2020Article
- Engineering Improves Enzymatic Synthesis of L-Tryptophan by Tryptophan Synthase fromMicroorganisms · 2020Article
- Learning the pattern of epistasis linking genotype and phenotype in a protein.Nature communications · 2019Article
- Structure-based switch of regioselectivity in the flavin-dependent tryptophan 6-halogenase Thal.The Journal of biological chemistry · 2019Article
- Understanding and Improving the Activity of Flavin-Dependent Halogenases via Random and Targeted Mutagenesis.Annual review of biochemistry · 2018Review
- Aromatic Halogenation by Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes.Chembiochem : a European journal of chemical biology · 2017Article
- Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering.ACS synthetic biology · 2017Article
- Dissecting the low catalytic capability of flavin-dependent halogenases.The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Directed evolution is a powerful tool for optimizing enzymes, and mutagenesis methods that improve enzyme library quality can significantly expedite the evolution process. Here, we report a simple method for targeted combinatorial codon mutagenesis (CCM). To demonstrate the utility of this method for protein engineering, CCM libraries were constructed for cytochrome P450
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.