ArticlePloS one2016
Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- Salmonid Rickettsial Septicemia (SRS) disease dynamics and Atlantic salmon immune response to Piscirickettsia salmonis LF-89 and EM-90 co-infection.Veterinary research · 2024Article
- Live and inactivatedFrontiers in immunology · 2023Article
- Transcriptomic Response of the Diazotrophic BacteriaInternational journal of molecular sciences · 2022Article
- Stress response and virulence factors in bacterial pathogens relevant for Chilean aquaculture: current status and outlook of our knowledge.Biological research · 2022Review
- Collective behavior and virulence arsenal of the fish pathogenFrontiers in cellular and infection microbiology · 2022Article
- Why DoesFrontiers in immunology · 2022Review
- PAMPs ofFrontiers in immunology · 2022Article
- RNA-Seq-Based Analysis of Cortisol-Induced Differential Gene Expression Associated withAnimals : an open access journal from MDPI · 2021Article
- Nutrient Scarcity in a New Defined Medium Reveals Metabolic Resistance to Antibiotics in the Fish PathogenFrontiers in microbiology · 2021Article
- Protein-Based Vaccine Protect AgainstFrontiers in immunology · 2021Article
- Global Proteomic Profiling ofMicroorganisms · 2020Article
- Article
- Applications of omics and nanotechnology to improve pig embryo production in vitro.Molecular reproduction and development · 2019Review
- Intracellular Bacterial Infections: A Challenge for Developing Cellular Mediated Immunity Vaccines for Farmed Fish.Microorganisms · 2018Review
- Dual RNA-Seq Uncovers Metabolic Amino Acids Dependency of the Intracellular BacteriumFrontiers in microbiology · 2018Article
- Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis.Proteomes · 2017Article
- Nutritional Immunity Triggers the Modulation of Iron Metabolism Genes in the Sub-Antarctic NotothenioidFrontiers in immunology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular facultative bacteria Piscirickettsia salmonis is one of the most important pathogens of the Chilean aquaculture. However, there is a lack of information regarding the whole genomic transcriptional response according to different extracellular environments. We used next generation sequencing (NGS) of RNA (RNA-seq) to study the whole transcriptome of an isolate of P. salmonis (FAVET-INBIOGEN) using a cell line culture and a modified cell-free liquid medium, with or without iron supplementation. This was done in order to obtain information about the factors there are involved in virulence and iron acquisition. First, the isolate was grown in the Sf21 cell line; then, the bacteria were cultured into a cell-free liquid medium supplemented or not with iron. We identified in the transcriptome, genes associated with type IV secretion systems, genes related to flagellar structure assembly, several proteases and sigma factors, and genes related to the development of drug resistance. Additionally, we identified for the first time several iron-metabolism associated genes including at least two iron uptake pathways (ferrous iron and ferric iron uptake) that are actually expressed in the different conditions analyzed. We further describe putative genes that are related with the use and storage of iron in the bacteria, which have not been previously described. Several sets of genes related to virulence were expressed in both the cell line and cell-free culture media (for example those related to flagellar structure; such as basal body, MS-ring, C-ring, proximal and distal rod, and filament), which may play roles in other basic processes rather than been restricted to virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.