ArticleForensic science, medicine, and pathology2017
Estimation of the human postmortem interval using an established rat mathematical model and multi-RNA markers.
Article in Forensic science, medicine, and pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 3 of them syntheses that pooled it.
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Who cites it
26 citing papers in PubMed, 3 syntheses or guidelines pooled it, 63 citations in OpenAlex.
- microRNAs as New Biomolecular Markers to Estimate Time since Death: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Potential Role of mRNA in Estimating Postmortem Interval: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- A small RNA, microRNA as a potential biomolecular marker to estimate post mortem interval in forensic science: a systematic review.International journal of legal medicine · 2023Pooled it
- Endogenous and Exogenous Small RNA Signatures as Novel Tools for Postmortem Interval Determination.Biomolecules · 2026Article
- Myocardium miRNA Analysis Reveals Potential Biomarkers of Sudden Coronary Death in Rats.Current issues in molecular biology · 2025Article
- Circular RNA circFat3 as a biomarker for construction of postmortem interval Estimation models in mouse brain tissues at multiple temperatures.Scientific reports · 2025Article
- Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals.Non-coding RNA · 2024Article
- MiR-122, miR-133a, and miR-206 as potential biomarkers for post-mortem interval estimation.Genes & genomics · 2024Article
- An experimental study to estimate the early postmortem interval based on the degradation of lncRNAs in rat brain tissue.Scientific reports · 2024Article
- Research progress and potential application of microRNA and other non-coding RNAs in forensic medicine.International journal of legal medicine · 2024Review
- Review
- The potential of using non-coding RNAs in forensic science applications.Forensic sciences research · 2023Review
- Review of the current and potential use of biological and molecular methods for the estimation of the postmortem interval in animals and humans.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Review
- Article
- Systematic Review on Post-Mortem Protein Alterations: Analysis of Experimental Models and Evaluation of Potential Biomarkers of Time of Death.Diagnostics (Basel, Switzerland) · 2022Review
- Circulating microRNAs as predictive biomarkers of coronary artery diseases in type 2 diabetes patients.Journal of clinical laboratory analysis · 2022Review
- Combining with lab-on-chip technology and multi-organ fusion strategy to estimate post-mortem interval of rat.Frontiers in medicine · 2022Article
- Postmortem expression of apoptosis-related genes in the liver of mice and their use for estimation of the time of death.International journal of legal medicine · 2021Article
- MicroRNAs as Useful Tools to Estimate Time Since Death. A Systematic Review of Current Literature.Diagnostics (Basel, Switzerland) · 2021Review
- Estimating the Postmortem Interval of Carcasses in the Water Using the Carrion Insect, Brain Tissue RNA, Bacterial Biofilm, and Algae.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our previous study, a R code-based mathematical model using RNA degradation patterns was developed for PMI determination in rat brain specimens. However, the postmortem changes of RNA are much more complicated in real cases, and there is still a huge challenge in efficiently applying information in animal data to real cases. In the present study, different RNA markers in both rat and human tissues were collected to screen valid biomarkers and the corresponding mathematical models were established and validated. With the same methodology, multi-RNA markers of myocardium and liver tissues were detected by qPCR and the Ct values of ten biomarkers generally increased with prolonged PMIs. 5S, miR-1 and miR-133a were shown to be optimum reference biomarkers that were not affected by a PMI of up to 5 or more days; however, liver-specific miR-122 began to degrade under higher temperatures and only 5S was selected as an endogenous control in the liver. Among the tested target RNAs, similar to our previous study in brain tissue, β-actin (ΔCt) was found to exhibit the best correlation coefficient with PMI and was employed to build mathematical models using R software. Following validation, the relatively low estimated error demonstrated that PMIs can be accurately predicted in human cases through comprehensive consideration of various factors and using effective biomarkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.