ArticleJournal of biomedical optics2016
Direct comparison between confocal and multiphoton microscopy for rapid histopathological evaluation of unfixed human breast tissue.
Article in Journal of biomedical optics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.Journal of the American Academy of Dermatology · 2026Article
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- Deep learning-enabled multiphoton microscopy predicts colorectal cancer recurrence from routine FFPE specimens.NPJ digital medicine · 2025Article
- Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT.Npj imaging · 2025Article
- Modeling intratumor heterogeneity in breast cancer.Biofabrication · 2024Review
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- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Hyperspectral dark-field microscopy of human breast lumpectomy samples for tumor margin detection in breast-conserving surgery.Journal of biomedical optics · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
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- Harnessing the power of optical microscopy for visualization and analysis of histopathological images.Biomedical optics express · 2023Review
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
- Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope.Journal of visualized experiments : JoVE · 2023Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Value of multiphoton microscopy in uro-oncology: a narrative review.Translational andrology and urology · 2023Review
- Tissue harvest with a laser microbiopsy.Journal of biomedical optics · 2022Article
- Is Real-Time Microscopy on the Horizon? A Brief Review of the Potential Future Directions in Clinical Breast Tumor Microscopy Implementation.Virchows Archiv : an international journal of pathology · 2022Review
- Rapid histological imaging of bone without microtome sectioning using nonlinear microscopy.Bone · 2022Article
- Multimodal Optical Coherence Tomography for Intraoperative Evaluation of Tumor Margins and Surgical Margins in Breast-Conserving Surgery.Sovremennye tekhnologii v meditsine · 2022Article
- Ex vivo confocal microscopy performs real-time assessment of renal biopsy in non-neoplastic diseases.Journal of nephrology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Rapid histopathological examination of surgical specimen margins using fluorescence microscopy during breast conservation therapy has the potential to reduce the rate of positive margins on postoperative histopathology and the need for repeat surgeries. To assess the suitability of imaging modalities, we perform a direct comparison between confocal fluorescence microscopy and multiphoton microscopy for imaging unfixed tissue and compare to paraffin-embedded histology. An imaging protocol including dual channel detection of two contrast agents to implement virtual hematoxylin and eosin images is introduced that provides high quality imaging under both one and two photon excitation. Corresponding images of unfixed human breast tissue show that both confocal and multiphoton microscopy can reproduce the appearance of conventional histology without the need for physical sectioning. We further compare normal breast tissue and invasive cancer specimens imaged at multiple magnifications, and assess the effects of photobleaching for both modalities using the staining protocol. The results demonstrate that confocal fluorescence microscopy is a promising and cost-effective alternative to multiphoton microscopy for rapid histopathological evaluation of ex vivo breast tissue.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.