Evidence map›Paper›PMID 28031943›Full record

ArticleFuture science OA2016

Supramolecular polymer formation by cyclic dinucleotides and intercalators affects dinucleotide enzymatic processing.

Shizuka Nakayama, Jie Zhou, Yue Zheng, Henryk Szmacinski, Herman O Sintim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Future science OA, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Intercepting second-messenger signaling by rationally designed peptides sequestering c-di-GMP.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  4. Cyclic Dinucleotides in Oral Bacteria and in Oral Biofilms.Frontiers in cellular and infection microbiology · 2017
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Shizuka NakayamaDepartment of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
Jie ZhouDepartment of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Yue ZhengDepartment of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Henryk SzmacinskiDepartment of Biochemistry & Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA; Department of Biochemistry & Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA.
Herman O SintimDepartment of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Purdue University West Lafayette · USUniversity of Maryland, College Park · USUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine. METHODS &

resultsAtomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA. The stability of c-di-GMP supramolecular polymers is dependent on both the nature of the cation present and the intercalator. C-di-GMP or cyclic diadenylic acid/intercalator complexes are more resistant to cleavage by YybT, a phosphodiesterase, than the uncomplexed nucleotides.

conclusionCleavage of bacterial cyclic dinucleotides could be slowed down via complexation with small molecules and that this could be utilized for diverse applications in nanotechnology and medicine.

Indexed as

c-di-AMPc-di-GMPfluorescence lifetimePDE inhibitionpolymersecond messengersmall moleculesupramolecular

Identifiers

PMID28031943
PMCPMC5137846
OpenAlexW2255934581

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.