Evidence map›Paper›PMID 28031892›Full record

ArticleFuture science OA2015

Selective nuclear localization of siRNA by metallic versus semiconducting single wall carbon nanotubes in keratinocytes.

John Torin Huzil, Evi Saliaj, Marina V Ivanova, Marjan Gharagozloo, Maria Jimena Loureiro, Constanze Lamprecht, Andreas Korinek, Ding Wen Chen, Marianna Foldvari

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Future science OA, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

John Torin HuzilSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Evi SaliajSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Marina V IvanovaSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Marjan GharagozlooSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Maria Jimena LoureiroSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Constanze LamprechtSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Andreas KorinekSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; Canadian Centre for Electron Microscopy, McMaster University, 1280 Main St. W, Hamilton, ON L8S 4L8, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; Canadian Centre for Electron Microscopy, McMaster University, 1280 Main St. W, Hamilton, ON L8S 4L8, Canada.
Ding Wen ChenSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Marianna FoldvariSchool of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada; School of Pharmacy, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe potential use of carbon nanotubes (CNTs) in gene therapy as delivery systems for nucleic acids has been recently recognized. Here, we describe that metallic versus semiconducting single-wall CNTs can produce significant differences in transfection rate and cellular distribution of siRNA in murine PAM212 keratinocytes. RESULTS/METHODOLOGY: The results of cell interaction studies, coupled with supportive computational simulations and ultrastructural studies revealed that the use of metallic single wall CNTs resulted in siRNA delivery into both the cytoplasm and nucleus of keratinocytes, whereas semiconducting CNTs resulted in delivery only to the cytoplasm.

conclusionUsing enriched fractions of metallic or semiconducting CNTs for siRNA complex preparation may provide specific subcellular targeting advantages.

Indexed as

chiralitydrug deliverykeratinocytesmetallic nanotubessemiconducting nanotubessingle wall carbon nanotubessiRNA transfection

Identifiers

PMID28031892
PMCPMC5137862

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.