ArticleGenes & development2016
Protein splicing of a recombinase intein induced by ssDNA and DNA damage.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 39 citations in OpenAlex.
- A protein chaperone can stabilize the intein-containing precursor to indirectly promote protein splicing.Protein science : a publication of the Protein Society · 2026Article
- Monitoring Protein Stability In Vivo Using an Intein-Based Biosensor.Bio-protocol · 2025Article
- Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication.Science advances · 2024Article
- Conditional protein splicing of the Mycobacterium tuberculosis RecA intein in its native host.Scientific reports · 2024Article
- Article
- Conditional protein splicing of thebioRxiv : the preprint server for biology · 2024Article
- An intein-based biosensor to measure protein stability in vivo.Protein science : a publication of the Protein Society · 2024Article
- Inteins-mechanism of protein splicing, emerging regulatory roles, and applications in protein engineering.Frontiers in microbiology · 2023Review
- A Chemical Biology Primer for NMR Spectroscopists.Journal of magnetic resonance open · 2022Article
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Conditional Alternative Protein Splicing Promoted by Inteins fromBiochemistry · 2022Article
- The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History.Frontiers in molecular biosciences · 2022Review
- Recruitment of Mobile Genetic Elements for Diverse Cellular Functions in Prokaryotes.Frontiers in molecular biosciences · 2022Review
- Reactive Chlorine Species Reversibly Inhibit DnaB Protein Splicing in Mycobacteria.Microbiology spectrum · 2021Article
- Construction and Quantitation of a Selectable Protein Splicing Sensor Using Gibson Assembly and Spot Titers.Current protocols · 2021Article
- Gold Nanoparticles Augment N-Terminal Cleavage and Splicing Reactions inFrontiers in bioengineering and biotechnology · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Article
- Allosteric Influence of Extremophile Hairpin Motif Mutations on the Protein Splicing Activity of a Hyperthermophilic Intein.Biochemistry · 2020Article
- PRP8 Intein in Onygenales: Distribution and Phylogenetic Aspects.Mycopathologia · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Inteins (or protein introns) autocatalytically excise themselves through protein splicing. We challenge the long-considered notion that inteins are merely molecular parasites and posit that some inteins evolved to regulate host protein function. Here we show substrate-induced and DNA damage-induced splicing, in which an archaeal recombinase RadA intein splices dramatically faster and more accurately when provided with ssDNA. This unprecedented example of intein splicing stimulation by the substrate of the invaded host protein provides compelling support in favor of inteins acting as pause buttons to arrest protein function until needed; then, an immediate activity switch is triggered, representing a new form of post-translational control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.