ArticleOrganogenesis2017
Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold.
Article in Organogenesis, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Decellularized liver scaffolds for constructing drug-metabolically functionalBioactive materials · 2025Article
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- Two Decades of Advances and Limitations in Organ Recellularization.Current issues in molecular biology · 2024Review
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- Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Design by Nature: Emerging Applications of Native Liver Extracellular Matrix for Cholangiocyte Organoid-Based Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2022Review
- Progress and Current Limitations of Materials for Artificial Bile Duct Engineering.Materials (Basel, Switzerland) · 2021Review
- 3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity.BioMed research international · 2020Article
- A Hepatic Scaffold from Decellularized Liver Tissue: Food for Thought.Biomolecules · 2019Review
- Directing the growth and alignment of biliary epithelium within extracellular matrix hydrogels.Acta biomaterialia · 2019Article
- Comparison of various reagents for preparing a decellularized porcine cartilage scaffold.American journal of translational research · 2019Article
- Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.Scientific reports · 2018Article
- In Vivo Performance of Decellularized Vascular Grafts: A Review Article.International journal of molecular sciences · 2018Review
- Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering.Journal of tissue engineeringArticle
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Authors and funding
18 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds using a human tumor cell line (HepG2, introduced via the bile duct). Subsequent studies were performed using either human tumor cells co-cultured with human umbilical vein endothelial cells (HUVECs, introduced via the portal vein) or rat neonatal cell slurry (introduced via the bile duct). Bio-chambers were used to circulate oxygenated growth medium via the portal vein at 37C for 5-7 days. Human HepG2 cells grew readily on the scaffold (n = 20). HepG2 cells co-cultured with HUVECs demonstrated viable human endothelial lining with concurrent hepatocyte growth (n = 10). In the series of neonatal cell slurry infusion (n = 10), distinct foci of neonatal hepatocytes were observed to repopulate the parenchyma of the scaffold. The presence of cholangiocytes was verified by CK-7 positivity. Quantitative albumin measurement from the grafts showed increasing albumin levels after seven days of perfusion. Graft albumin production was higher than that observed in traditional cell culture. This data shows that rat liver scaffolds support human cell ingrowth. The scaffold likewise supported the engraftment and survival of neonatal rat liver cell slurry. Recellularization of liver scaffolds thus presents a promising model for functional liver engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.