ArticlePlanta2017
Localization of RNS2 ribonuclease to the vacuole is required for its role in cellular homeostasis.
Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- An active RNA transport mechanism into plant vacuoles.Nucleic acids research · 2026Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Frontiers in plant RNA research in ICAR2023: from lab to innovative agriculture.Plant molecular biology · 2024Article
- RNS2 is required for the biogenesis of a wounding responsive 16 nts tsRNA in Arabidopsis thaliana.Plant molecular biology · 2024Article
- Autophagy: A New Avenue and Biochemical Mechanisms to Mitigate the Climate Change.Scientifica · 2024Review
- Metabolic footprints in phosphate-starved plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Extracellular RNA: mechanisms of secretion and potential functions.Journal of experimental botany · 2023Article
- Adaptive strategies of plants to conserve internal phosphorus under P deficient condition to improve P utilization efficiency.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
- Tissue-specific enhancement of OsRNS1 with root-preferred expression is required for the increase of crop yield.Journal of advanced research · 2022Article
- Intracellular phosphate recycling systems for survival during phosphate starvation in plants.Frontiers in plant science · 2022Review
- The mRNA-binding proteome of a critical phase transition during Arabidopsis seed germination.The New phytologist · 2022Article
- Organellar and Secretory Ribonucleases: Major Players in Plant RNA Homeostasis.Plant physiology · 2020Article
- Phosphate-Starvation-Inducible S-Like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay.Frontiers in plant science · 2020Article
- Review
- Protein and RNA Quality Control by Autophagy in Plant Cells.Molecules and cells · 2019Review
- New advances in autophagy in plants: Regulation, selectivity and function.Seminars in cell & developmental biology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionLocalization of the RNase RNS2 to the vacuole via a C-terminal targeting signal is essential for its function in rRNA degradation and homeostasis. RNase T2 ribonucleases are highly conserved enzymes present in the genomes of nearly all eukaryotes and many microorganisms. Their constitutive expression in different tissues and cell types of many organisms suggests a housekeeping role in RNA homeostasis. The Arabidopsis thaliana class II RNase T2, RNS2, is encoded by a single gene and functions in rRNA degradation. Loss of RNS2 results in RNA accumulation and constitutive activation of autophagy, possibly as a compensatory mechanism. While the majority of RNase T2 enzymes is secreted, RNS2 is located within the vacuole and in the endoplasmic reticulum (ER), possibly within ER bodies. As RNS2 has a neutral pH optimum, and the endomembrane organelles are connected by vesicle transport, the site within the endomembrane system at which RNS2 functions is unclear. Here we demonstrate that localization to the vacuole is essential for the physiological function of RNS2. A mutant allele of RNS2, rns2-1, results in production of an active RNS2 RNase but with a mutation that removes a putative C-terminal vacuolar targeting signal. The mutant protein is, therefore, secreted from the cell. This results in a constitutive autophagy phenotype similar to that observed in rns2 null mutants. These findings illustrate that the intracellular retention of RNS2 and localization within the vacuole are critical for its cellular function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.