ArticleNucleic acids research2017
Salt-mediated two-site ligand binding by the cocaine-binding aptamer.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- Insulin Quantification Through Electrochemical and Optical Aptasensors: A Review.Annals of biomedical engineering · 2025Review
- Article
- Interactions of Ligand, Aptamer, and Complementary Oligonucleotide: Studying Impacts of NaMolecules (Basel, Switzerland) · 2025Article
- Rapid nanomolar detection of cocaine in biofluids by electrochemical aptamer-based sensor with low-temperature effect for drugged driving screening.Mikrochimica acta · 2024Article
- Aptamer-based nanotrains and nanoflowers as quinine delivery systems.International journal of pharmaceutics: X · 2023Article
- Aptamers as Theragnostic Tools in Prostate Cancer.Biomolecules · 2022Review
- Unraveling the binding mode of a methamphetamine aptamer: A spectroscopic and calorimetric study.Biophysical journal · 2022Article
- DNA binding by the antimalarial compound artemisinin.Scientific reports · 2022Article
- Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.ACS applied materials & interfaces · 2021Article
- Thermodynamic analysis of cooperative ligand binding by the ATP-binding DNA aptamer indicates a population-shift binding mechanism.Scientific reports · 2020Article
- Reduction in Dynamics of Base pair Opening upon Ligand Binding by the Cocaine-Binding Aptamer.Biophysical journal · 2020Article
- Structure-switching fluorescence aptasensor for sensitive detection of chloramphenicol.Mikrochimica acta · 2020Article
- 7,8-Dihydro-8-oxoguanosine Lesions Inhibit the Theophylline Aptamer or Change Its Selectivity.Chembiochem : a European journal of chemical biology · 2020Article
- A Bottom-Up Approach for Developing Aptasensors for Abused Drugs: Biosensors in Forensics.Biosensors · 2019Review
- A proof of concept application of aptachain: ligand-induced self-assembly of a DNA aptamer.RSC advances · 2019Article
- G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host Cells.Molecular therapy. Nucleic acids · 2018Article
- Comparison of the free and ligand-bound imino hydrogen exchange rates for the cocaine-binding aptamer.Journal of biomolecular NMR · 2017Article
- Are there two different binding sites for ATP on the myosin head, or only one that switches between two conformers?Journal of muscle research and cell motility · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multisite ligand binding by proteins is commonly utilized in the regulation of biological systems and exploited in a range of biochemical technologies. Aptamers, although widely utilized in many rationally designed biochemical systems, are rarely capable of multisite ligand binding. The cocaine-binding aptamer is often used for studying and developing sensor and aptamer-based technologies. Here, we use isothermal titration calorimetry (ITC) and NMR spectroscopy to demonstrate that the cocaine-binding aptamer switches from one-site to two-site ligand binding, dependent on NaCl concentration. The high-affinity site functions at all buffer conditions studied, the low-affinity site only at low NaCl concentrations. ITC experiments show the two ligand-binding sites operate independently of one another with different affinities and enthalpies. NMR spectroscopy shows the second binding site is located in stem 2 near the three-way junction. This ability to control ligand binding at the second site by adjusting the concentration of NaCl is rare among aptamers and may prove a useful in biotechnology applications. This work also demonstrates that in vitro selected biomolecules can have functions as complex as those found in nature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.