ArticleNature methods2017
RESA identifies mRNA-regulatory sequences at high resolution.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
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- NaP-TRAP reveals the regulatory grammar in 5'UTR-mediated translation regulation during zebrafish development.Nature communications · 2024Article
- Decoding biology with massively parallel reporter assays and machine learning.Genes & development · 2024Review
- UPF1 regulates mRNA stability by sensing poorly translated coding sequences.Cell reports · 2024Article
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- mRNA 5' terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay.PLoS genetics · 2022Article
- Engineering Alendronate-Composed Iron Nanochelator for Efficient Peritoneal Carcinomatosis Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Modulation of miRISC-Mediated Gene Silencing in Eukaryotes.Frontiers in molecular biosciences · 2022Review
- Spatio-temporal mRNA tracking in the early zebrafish embryo.Nature communications · 2021Article
- Crosstalk between codon optimality and cis-regulatory elements dictates mRNA stability.Genome biology · 2021Article
- RNA structure-wide discovery of functional interactions with multiplexed RNA motif library.Nature communications · 2020Article
- Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole.International journal of molecular sciences · 2020Article
- CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.Nucleic acids research · 2020Article
- MaveDB: an open-source platform to distribute and interpret data from multiplexed assays of variant effect.Genome biology · 2019Article
- High-Throughput Quantification ofHuman gene therapy · 2019Article
- Genome wide analysis of 3' UTR sequence elements and proteins regulating mRNA stability during maternal-to-zygotic transition in zebrafish.Genome research · 2019Article
- Functional assays for transcription mechanisms in high-throughput.Methods (San Diego, Calif.) · 2019Review
- Mechanistic Insights into MicroRNA-Mediated Gene Silencing.Cold Spring Harbor perspectives in biology · 2019Review
- Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.Nature structural & molecular biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA-element selection assay (RESA), a method that selects RNA elements on the basis of their activity in vivo and uses high-throughput sequencing to provide a quantitative measurement of their regulatory functions at near-nucleotide resolution. We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. RESA provides a sensitive and quantitative measure of microRNA activity in vivo and also identifies novel regulatory sequences. To uncover specific sequence requirements within regulatory elements, we developed a bisulfite-mediated nucleotide-conversion strategy for large-scale mutational analysis (RESA-bisulfite). Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.