ArticleNature structural & molecular biology2017
Crystal structure of the MOP flippase MurJ in an inward-facing conformation.
Article in Nature structural & molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 87 citations in OpenAlex.
- Structures of the lipid II flippase from the monoderm pathogen Staphylococcus aureus.The Journal of biological chemistry · 2026Article
- Mechanistic basis of teichoic acid transport by a gatekeeper flippase.Nature communications · 2026Article
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- Targeting Bacterial Cell Wall Synthesis: Structural Insights and Emerging Therapeutic Strategies.Pharmaceutics · 2026Review
- Phage lysis protein LysScience advances · 2025Article
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- Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide.Open biology · 2025Article
- Rft1 catalyzes lipid-linked oligosaccharide translocation across the ER membrane.Nature communications · 2024Article
- Heterogenous Expression and Purification of Lipid II Flippase fromProtein and peptide letters · 2024Article
- The fall of the mycobacterial cell wall: interrogating peptidoglycan synthesis for novel anti-TB agents.PeerJ · 2024Review
- Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry.Nature communications · 2023Article
- Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins.Nature chemical biology · 2023Article
- Article
- Targeting Essential Hypothetical Proteins ofBioMed research international · 2023Article
- Lipid Transport Across Bacterial Membranes.Annual review of cell and developmental biology · 2022Review
- Structure and Mechanism of the Lipid Flippase MurJ.Annual review of biochemistry · 2022Review
- XAC4296 Is a Multifunctional and ExclusiveMicroorganisms · 2022Article
- The Bacterial Cell Wall: From Lipid II Flipping to Polymerization.Chemical reviews · 2022Review
- Weaving of bacterial cellulose by the Bcs secretion systems.FEMS microbiology reviews · 2022Review
- Sampling alternative conformational states of transporters and receptors with AlphaFold2.eLife · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Peptidoglycan (PG) protects bacteria from osmotic lysis, and its biogenesis is a key antibiotic target. A central step in PG biosynthesis is flipping of the lipid-linked PG precursor lipid II across the cytoplasmic membrane for subsequent incorporation into PG. MurJ, part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) transporter superfamily, was recently shown to carry out this process. However, understanding of how MurJ flips lipid II, and of how MOP transporters operate in general, remains limited due to a lack of structural information. Here we present a crystal structure of MurJ from Thermosipho africanus in an inward-facing conformation at 2.0-Å resolution. A hydrophobic groove is formed by two C-terminal transmembrane helices, which leads into a large central cavity that is mostly cationic. Our studies not only provide the first structural glimpse of MurJ but also suggest that alternating access is important for MurJ function, which may be applicable to other MOP superfamily transporters.
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