ArticleMolecular microbiology2017
Relaxation of DNA supercoiling leads to increased invasion of epithelial cells and protein secretion by Campylobacter jejuni.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- An invasive phenotype induced by relaxation of DNA supercoiling inMicrobiology (Reading, England) · 2025Article
- Different stages of the infection cycle are enriched forMicrobiology (Reading, England) · 2023Article
- The Missing Pieces: The Role of Secretion Systems inBiomolecules · 2023Review
- Relevance and Importance of Biofilms in the Resistance and Spreading of Campylobacter spp. Within the Food Chain.Advances in experimental medicine and biology · 2023Article
- Genomic Screening of Antimicrobial Resistance Markers in UK and USAntimicrobial agents and chemotherapy · 2022Article
- Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA-mimic protein and may be involved in the adaptation of Burkholderia cenocepacia.MicrobiologyOpen · 2022Article
- The bile salt deoxycholate inducesFrontiers in microbiology · 2022Article
- A novel high-content screening approach for the elucidation of C. jejuni biofilm composition and integrity.BMC microbiology · 2021Article
- Epidemiologic Associations Vary Between Tetracycline and Fluoroquinolone ResistantFrontiers in public health · 2021Article
- Antimicrobial Resistance and Virulence Properties ofAnimals : an open access journal from MDPI · 2020Article
- Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni.Scientific reports · 2019Article
- Comparison of genomes and proteomes of four whole genome-sequenced Campylobacter jejuni from different phylogenetic backgrounds.PloS one · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Invasion of intestinal epithelial cells by Campylobacter jejuni is a critical step during infection of the intestine by this important human pathogen. In this study we investigated the role played by DNA supercoiling in the regulation of invasion of epithelial cells and the mechanism by which this could be mediated. A significant correlation between more relaxed DNA supercoiling and an increased ability of C. jejuni strains to penetrate human epithelial cells was demonstrated. Directly inducing relaxation of DNA supercoiling in C. jejuni was shown to significantly increase invasion of epithelial cells. Mutants in the fibronectin binding proteins CadF and FlpA still displayed an increased invasion after treatment with novobiocin suggesting these proteins were not essential for the observed phenotype. However, a large increase in protein secretion from multiple C. jejuni strains upon relaxation of DNA supercoiling was demonstrated. This increase in protein secretion was not mediated by outer membrane vesicles and appeared to be dependent on an intact flagellar structure. This study identifies relaxation of DNA supercoiling as playing a key role in enhancing C. jejuni pathogenesis during infection of the human intestine and identifies proteins present in a specific invasion associated secretome induced by relaxation of DNA supercoiling.
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