ArticleBiomedical optics express2016
Efficient multi-site two-photon functional imaging of neuronal circuits.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Two-site microendoscopic imaging probe for simultaneous three-dimensional imaging at two anatomic locations in tissues.Optics letters · 2024Article
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Localization of Fluorescent Targets in Deep Tissue With Expanded Beam Illumination for Studies of Cancer and the Brain.IEEE transactions on medical imaging · 2020Article
- High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning.Cell reports · 2020Article
- Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography.Biomedical optics express · 2020Article
- 3D Localization for Light-Field Microscopy via Convolutional Sparse Coding on Epipolar Images.IEEE transactions on computational imaging · 2020Article
- High speed functional imaging with source localized multifocal two-photon microscopy.Biomedical optics express · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Two-photon imaging using high-speed multi-channel detectors is a promising approach for optical recording of cellular membrane dynamics at multiple sites. A main bottleneck of this technique is the limited number of photons captured within a short exposure time (~1ms). Here, we implement temporal gating to improve the two-photon fluorescence yield from holographically projected multiple foci whilst maintaining a biologically safe incident average power. We observed up to 6x improvement in the signal-to-noise ratio (SNR) in Fluorescein and cultured hippocampal neurons showing evoked calcium transients. With improved SNR, we could pave the way to achieving multi-site optical recording of fluorogenic probes with response times in the order of ~1ms.
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Registered trials
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