Evidence map›Paper›PMID 28018729›Full record

ArticleBiomedical optics express2016

Volumetric optical mapping in early embryonic hearts using light-sheet microscopy.

Pei Ma, Dennis C Chan, Shi Gu, Michiko Watanabe, Michael W Jenkins, Andrew M Rollins

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024
    Review
  2. Review
  3. Article
  4. Article
  5. Optical Electrophysiology in the Developing Heart.Journal of cardiovascular development and disease · 2018
    Review
  6. Article
  7. A review of optical pacing with infrared light.Journal of neural engineering · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Pei MaDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA; Shanghai Key Laboratory of Modern Optical Systems, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Dennis C ChanDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Shi GuDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Michiko WatanabeDepartment of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Michael W JenkinsDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA; Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Andrew M RollinsDepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Case Western Reserve University · US

Funding

Optical Tools to Assess the Role of Hemodynamics in the Development of Congenital Heart DefectsR01HL126747 · NHLBI · CASE WESTERN RESERVE UNIVERSITY · PI JENKINS, MICHAEL W. · 2015 to 2025
$6.4M
OCT Imaging Technology for Developmental Cardiology ResearchR01HL083048 · NHLBI · CASE WESTERN RESERVE UNIVERSITY · PI ROLLINS, ANDREW MARTIN · 2006 to 2016
$3.2M
Optical pacing of the embryonic heartR21HL115373 · NHLBI · CASE WESTERN RESERVE UNIVERSITY · PI JENKINS, MICHAEL W. · 2012 to 2013
$422k
NHLBI NIH HHS R01 HL083048NHLBI NIH HHS R01 HL126747NHLBI NIH HHS R21 HL115373
6 · The paper itself

Abstract

Optical mapping (OM) of electrical activity using voltage-sensitive fluorescent dyes is a powerful tool for the investigation of embryonic cardiac electrophysiology. However, because conventional OM integrates the signal in depth and projects it to a two-dimensional plane, information acquired is incomplete and dependent upon the orientation of the sample. This complicates interpretation of data, especially when comparing one heart to another. To overcome this limitation, we present volumetric OM using light-sheet microscopy, which enables high-speed capture of optically sectioned slices. Voltage-sensitive fluorescence images from multiple planes across entire early embryonic quail hearts were acquired, and complete, orientation-independent, four-dimensional maps of transmembrane potential are demonstrated. Volumetric OM data were collected while using optical pacing to control the heart rate, paving the way for physiological measurements and precise manipulation of the heartbeat in the future.

Indexed as

(110.0110) Imaging systems(170.2520) Fluorescence microscopy(170.2655) Functional monitoring and imaging(170.3880) Medical and biological imaging(170.6900) Three-dimensional microscopy

Identifiers

PMID28018729
PMCPMC5175556
OpenAlexW2551091715

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.